KBTBD2 Monoclonal antibody, PBS Only

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  WB analysis using 67895-1-Ig (same clone as 67895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67895-1-Ig (same clone as 67895-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67895-1-Ig (same clone as 67895-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KBTBD2 antibody (67895-1-Ig, Clone: 2A9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue). This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

KBTBD2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67895-1-PBS

Clone No.2A9F3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BKLHD1, KBTBD2, KIAA1489

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Product Information

67895-1-PBS targets KBTBD2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4933

  Product name: Recombinant human KBTBD2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-349 aa of BC047107

  Sequence: MSTQDERQINTEYAVSLLEQLKLFYEQQLFTDIVLIVEGTEFPCHKMVLATCSSYFRAMFMSGLSESKQTHVHLRNVDAATLQIIITYAYTGNLAMNDSTVEQLYETACFLQVEDVLQRCREYLIKKINAENCVRLLSFADLFSCEELKQSAKRMVEHKFTAVYHQDAFMQLSHDLLIDILSSDNLNVGKEETVREAAMLWLEYNTESRSQYLSSVLSQIRIDALSEVTQRAWFQGLPPNDKSVVVQGLYKSMRKFFKPRLGMTKEEMMIFIEASSENPCSLYSSVCYSPQAEKVYKLCSRPADLHKVGTVVTPDNDIYIAGGQVPLKNTKTNHSKTSKLQTAFRTVNC

• Full Name: kelch repeat and BTB (POZ) domain containing 2
• Calculated Molecular Weight: 622 aa, 71 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC047107
• Gene Symbol: KBTBD2
• Gene ID (NCBI): 25948
• RRID: AB_2918651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8IY47
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106).