Kv1.2 Polyclonal antibody

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  WB analysis using 14235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14235-1-AP (Kv1.2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14235-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14235-1-AP (Kv1.2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells/tissue using 14235-1-AP

  WB result of anti-KCNA2 (14235-1-AP) in different cell and tissue lysates.

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  IP experiment of HeLa using 14235-1-AP

  IP result of anti-Kv1.2 (IP:14235-1-AP, 4ug; Detection:14235-1-AP 1:500) with HeLa cells lysate 5200ug.

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  IHC staining of mouse brain using 14235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14235-1-AP (Kv1.2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14235-1-AP (Kv1.2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Kv1.2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14235-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

HBK5, HK4, HUKIV, KCNA2, KV1.2, MK2, NGK1, RBK2

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Tested Applications

• Positive WB detected in: A549 cells, multi-cells/tissue, human heart tissue, HeLa cells, HepG2 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

14235-1-AP targets Kv1.2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5477

  Product name: Recombinant human KCNA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-163 aa of BC043564

  Sequence: MTVATGDPADEAAALPGHPQDTYDPEADHECCERVVINISGLRFETQLKTLAQFPETLLGDPKKRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLRRPVNVPLDIFSEEIRFYELGEEAMEMFREDEGYIKEEERPLPENEFQRQVWLLFEYPESSGPAR

• Full Name: potassium voltage-gated channel, shaker-related subfamily, member 2
• Calculated Molecular Weight: 499 aa, 57 kDa
• Observed Molecular Weight: 66-75 kDa
• GenBank Accession Number: BC043564
• Gene Symbol: Kv1.2
• Gene ID (NCBI): 3737
• RRID: AB_2280576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16389
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Adv Sci (Weinh); Branched-Chain Amino Acids Deficiency Promotes Diabetic Neuropathic Pain Through Upregulating LAT1 and Inhibiting Kv1.2 Channel; Authors - Ze-Yu Zhou
human	WB	Cell Commun Signal; Transcriptional regulation of KCNA2 coding Kv1.2 by EWS::FLI1: involvement in controlling the YAP/Hippo signalling pathway and cell proliferation; Authors - Maryne Dupuy