KCNAB2 Polyclonal antibody

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KCNAB2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17890-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AKR6A5, HKvbeta2, HKvbeta2.1, HKvbeta2.2, K(+) channel subunit beta 2, KCNA2B, KCNAB2, KCNK2, KV BETA 2, TREK, TREK1

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  WB analysis of mouse brain using 17890-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 17890-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 17890-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells
• Positive IHC detected in: mouse brain tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17890-1-AP targets KCNAB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12096

  Product name: Recombinant human KCNAB2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-395 aa of BC110351

  Sequence: MYPESTTGSPARLSLRQTGSPGMIYSTRYGSPKRQLQFYRNLGKSGLRVSCLGLGTWVTFGGQITDEMAEQLMTLAYDNGINLFDTAEVYAAGKAEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGLKASLERLQLEYVDVVFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLTPPICEQAEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDSGIPPYSRASLKGYQWLKDKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASNADQLMENIGAIQVRVRGPAGQRAHPSPSPVQCILPGSSCVPGSVLGTQDAPVNHQSCAPGELAFQQEQT

• Full Name: potassium voltage-gated channel, shaker-related subfamily, beta member 2
• Calculated Molecular Weight: 395 aa, 44 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC110351
• Gene Symbol: KCNAB2
• Gene ID (NCBI): 8514
• RRID: AB_2249546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13303
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The KCNAB2 Polyclonal antibody (Cat# 17890-1-AP) has 4 total citations. These appear in Cells, Scientific Reports, Acta Biochimica et Biophysica Sinica, and Brain Sciences. The associated research fields include lung adenocarcinoma prognosis and immune infiltration, neuronal activity and hyperexcitability states, renal cancer autoantibody biomarker screening, and valproic acid-induced protein expression in neuroblastoma cells.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cells; The Ion Channel Gene KCNAB2 Is Associated with Poor Prognosis and Loss of Immune Infiltration in Lung Adenocarcinoma; Authors - Yin Lyu
	IHC	Sci Rep; The long non-coding RNA NEAT1 is responsive to neuronal activity and is associated with hyperexcitability states.; Authors - Guy Barry
human	WB	Acta Biochim Biophys Sin (Shanghai); Screening of autoantibodies as biomarkers in the serum of renal cancer patients based on human proteome microarray; Authors - Yangyang Sun
human	WB	Brain Sci; Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells.; Authors - Tsung-Ming Hu