KCNAB2 Polyclonal antibody

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  WB analysis of mouse brain using 17890-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17890-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17890-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KCNAB2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17890-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AKR6A5, HKvbeta2, HKvbeta2.1, HKvbeta2.2, K(+) channel subunit beta 2, KCNA2B, KCNAB2, KCNK2, KV BETA 2, TREK, TREK1

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells
• Positive IHC detected in: mouse brain tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

17890-1-AP targets KCNAB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12096

  Product name: Recombinant human KCNAB2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-395 aa of BC110351

  Sequence: MYPESTTGSPARLSLRQTGSPGMIYSTRYGSPKRQLQFYRNLGKSGLRVSCLGLGTWVTFGGQITDEMAEQLMTLAYDNGINLFDTAEVYAAGKAEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGLKASLERLQLEYVDVVFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLTPPICEQAEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDSGIPPYSRASLKGYQWLKDKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASNADQLMENIGAIQVRVRGPAGQRAHPSPSPVQCILPGSSCVPGSVLGTQDAPVNHQSCAPGELAFQQEQT

• Full Name: potassium voltage-gated channel, shaker-related subfamily, beta member 2
• Calculated Molecular Weight: 395 aa, 44 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC110351
• Gene Symbol: KCNAB2
• Gene ID (NCBI): 8514
• RRID: AB_2249546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13303
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IHC	Sci Rep; The long non-coding RNA NEAT1 is responsive to neuronal activity and is associated with hyperexcitability states.; Authors - Guy Barry
human	WB	Brain Sci; Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells.; Authors - Tsung-Ming Hu
human	WB	Cells; The Ion Channel Gene KCNAB2 Is Associated with Poor Prognosis and Loss of Immune Infiltration in Lung Adenocarcinoma; Authors - Yin Lyu
human	WB	Acta Biochim Biophys Sin (Shanghai); Screening of autoantibodies as biomarkers in the serum of renal cancer patients based on human proteome microarray; Authors - Yangyang Sun