KCNB1-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

KCNB1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19963-1-AP

Synonyms

DRK1, h DRK1, KCNB1, KCNB1-Specific, KV2.1

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 19963-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19963-1-AP (KCNB1-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19963-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19963-1-AP (KCNB1-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

19963-1-AP targets KCNB1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: potassium voltage-gated channel, Shab-related subfamily, member 1
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: ~130 kDa
• GenBank Accession Number: NM_004975
• Gene Symbol: KCNB1
• Gene ID (NCBI): 3745
• RRID: AB_2878631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCNB1, also named as DRK1, KV2.1, and h-DRK1, belongs to the potassium channel family and B (Shab) subfamily. KCNB1 mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient. The antibody is specific to KCNB1.

Publications

Species	Application	Title
human,rat	WB	Cardiovasc Ther; Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b.; Authors - Shuang Liang
rat	WB	Int J Clin Exp Med; Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1.; Authors - Wenjuan Liu
mouse	WB	Adv Sci (Weinh); Cdyl Deficiency Brakes Neuronal Excitability and Nociception through Promoting Kcnb1 Transcription in Peripheral Sensory Neurons.; Authors - Zhao-Wei Sun ; KD Validated
human	WB	J Cardiovasc Pharmacol; circNFXL1 Modulates the Kv2.1 Channel Function in Hypoxic Human Pulmonary Artery Smooth Muscle Cells via Sponging miR-29b-2-5p as a Competitive Endogenous RNA; Authors - Shan-Shan Li