KCNE4 Polyclonal antibody

KCNE4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18289-1-AP

Synonyms

KCNE4, MinK related peptide 3, MIRP3

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Validation Data Gallery

• 

  WB analysis of L02 using 18289-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18289-1-AP (KCNE4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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Tested Applications

• Positive WB detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

18289-1-AP targets KCNE4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: potassium voltage-gated channel, Isk-related family, member 4
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: NM_080671
• Gene Symbol: KCNE4
• Gene ID (NCBI): 23704
• RRID: AB_2132300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCNE4, also named as MIRP3 and MGC2035, is a membrane protein belongs to a family of single transmembrane domain proteins known to have dramatic effect on the gating of certain potassium channels. KCNE4 is expressed strongly in heart, skeletal muscle and kidney. Electrophysiological studies show that human KCNE4 modulates the activation of the KCNQ1 channel.

Publications

Species	Application	Title
human	WB,IF	J Cell Sci; The C-terminal domain of Kv1.3 regulates functional interactions with the KCNE4 subunit.; Authors - Laura Solé
human	WB	Sci Rep; Calmodulin-dependent KCNE4 dimerization controls membrane targeting.; Authors - Sara R Roig
human	IHC	Sci Rep; KCNE4-dependent functional consequences of Kv1.3-related leukocyte physiology.; Authors - Albert Vallejo-Gracia
human	WB,IF	FASEB J; The calmodulin-binding tetraleucine motif of KCNE4 is responsible for association with Kv1.3.; Authors - Laura Solé