Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
26426-1-AP targets KCNH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16647 Product name: Recombinant human KCNH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 686-850 aa of BC113709 Sequence: VKREEEERMKRKNEAPLILPPDHPVRRLFQRFRQQKEARLAAERGGRDLDDLDVEKGNVLTEHASANHSLVKASVVTVRESPATPVSFQAASTSGVPDHAKLQAPGSECLGPKGGGGDCAKRKSWARFKDACGKSEDWNKVSKAESMETLPERTKASGEATLKKT Predict reactive species |
| Full Name | potassium voltage-gated channel, subfamily H (eag-related), member 1 |
| Calculated Molecular Weight | 989 aa, 111 kDa |
| Observed Molecular Weight | 111 kDa |
| GenBank Accession Number | BC113709 |
| Gene Symbol | KCNH1 |
| Gene ID (NCBI) | 3756 |
| RRID | AB_3742187 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95259 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Potassium voltage-gated channel subfamily H member 1 (KCNH1, also known as Kv10.1 and EAG) a member of the EAG (ether-à-go-go) family, which is involved in intracellular signaling, cell proliferation, and tumorigenesis (PMID: 35639255). KCNH1 is mainly expressed in the adult central nervous system. It also plays a role in controlling K+ fux that regulates resting membrane potential in excitable and non-excitable cells and is activated at the onset of myoblast differentiation (PMID: 29333676; 27029528; 9738473).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KCNH1 antibody 26426-1-AP | Download protocol |
| IP protocol for KCNH1 antibody 26426-1-AP | Download protocol |
| WB protocol for KCNH1 antibody 26426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
