KCNH5 Polyclonal antibody

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  WB analysis using 17912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17912-1-AP (KCNH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

KCNH5 Polyclonal Antibody for WB, ELISA

Cat No. 17912-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

EAG2, H EAG2, hEAG2, KCNH5, Kv10.2

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17912-1-AP targets KCNH5 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12312

  Product name: Recombinant human KCNH5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 640-988 aa of BC073979

  Sequence: IIKREALLKVLDFYTAFANSFSRNLTLTCNLRKRIIFRKISDVKKEEEERLRQKNEVTLSIPVDHPVRKLFQKFKQQKELRNQGSTQGDPERNQLQVESRSLQNGTSITGTSVVTVSQITPIQTSLAYVKTSESLKQNNRDAMELKPNGGADQKCLKVNSPIRMKNGNGKGWLRLKNNMGAHEEKKEDWNNVTKAESMGLLSEDPKSSDSENSVTKNPLRKTDSCDSGITKSDLRLDKAGEARSPLEHSPIQADAKHPFYPIPEQALQTTLQEVKHELKEDIQLLSCRMTALEKQVAEILKILSEKSVPQASSPKSQMPLQVPPQIPCQDIFSVSRPESPESDKDEIHF

• Full Name: potassium voltage-gated channel, subfamily H (eag-related), member 5
• Calculated Molecular Weight: 988 aa, 112 kDa
• Observed Molecular Weight: 69-71 kDa
• GenBank Accession Number: BC073979
• Gene Symbol: KCNH5
• Gene ID (NCBI): 27133
• RRID: AB_3085546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NCM2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Potassium voltage-gated channel subfamily H member 5 (KCNH5), also known as Ether-a-go-go potassium channel 2 (hEAG2) belongs to mammalian potassium channel subfamily and is upregulated in a subset of Medulloblastoma (MB). KCNH5 protein is confined intracellularly during interphase but is enriched in the plasma membrane during late G2 phase and mitosis (PMID: 22855790, 26258683).