KCNH7 Polyclonal antibody

KCNH7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13622-1-AP

Synonyms

Eag related protein 3, ERG 3, ERG3, hERG 3, HERG3, KCNH7, Kv11.3

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Validation Data Gallery

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  WB analysis of human brain using 13622-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13622-1-AP (KCNH7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13622-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13622-1-AP (KCNH7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13622-1-AP

  IP result of anti-KCNH7 (IP:13622-1-AP, 4ug; Detection:13622-1-AP 1:300) with mouse brain tissue lysate 5200ug.

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  IHC staining of human heart using 13622-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13622-1-AP (KCNH7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13622-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13622-1-AP (KCNH7 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13622-1-AP targets KCNH7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCNH7 fusion protein Ag4532
• Full Name: potassium voltage-gated channel, subfamily H (eag-related), member 7
• Calculated Molecular Weight: 1196 aa, 135 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC035815
• Gene Symbol: KCNH7
• Gene ID (NCBI): 90134
• RRID: AB_10638620
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.