KCNIP3 Recombinant monoclonal antibody

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  WB analysis using 86579-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86579-1-RR (KCNIP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86579-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86579-1-RR against Human KCNIP3 were performed. The affinity constant is 0.903 nM.

KCNIP3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86579-1-RR

Clone No.251515A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

A-type potassium channel modulatory protein 3, Calsenilin, DRE-antagonist modulator, Kv channel-interacting protein 3

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86579-1-RR targets KCNIP3 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2665

  Product name: Recombinant human KCNIP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-256 aa of BC012850

  Sequence: MQPAKEVTKASDGSLLGDLGHTPLSKKEGIKWQRPRLSRQALMRCCLVKWILSSTAPQGSDSSDSELELSTVRHQPEGLDQLQAQTKFTKKELQSLYRGFKNECPTGLVDEDTFKLIYAQFFPQGDATTYAHFLFNAFDADGNGAIHFEDFVVGLSILLRGTVHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRHTYPILREDAPAEHVERFFEKMDRNQDGVVTIEEFLEACQKDENIMSSMQLFENVI

• Full Name: Kv channel interacting protein 3, calsenilin
• Calculated Molecular Weight: 256 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC012850
• Gene Symbol: KCNIP3
• Gene ID (NCBI): 30818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y2W7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCNIP3 also known as Calsenilin, CSEN, DREAM, or KCHIP3, is a protein-coding gene located on human chromosome 2q11.1 . It encodes a small calcium-binding protein belonging to the recoverin branch of the EF-hand superfamily. KCNIP3 is an integral subunit of native Kv4 channel complexes, regulating A-type potassium currents in response to intracellular calcium changes, thereby modulating neuronal excitability. KCNIP3 interacts with presenilin 1 (PSEN1) and presenilin 2 (PSEN2), influencing γ-secretase activity and β-amyloid formation, potentially linking it to Alzheimer's disease pathology.