Tested Applications
| Positive WB detected in | pig brain tissue, human brain tissue, rat brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60133-1-Ig targets KCNIP4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4863 Product name: Recombinant human KCNIP4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-250 aa of BC032520 Sequence: MNVRRVESISAQLEEASSTGGFLYAQNSTKRSIKERLMKLLPCSAAKTSSPAIQNSVEDELEMATVRHRPEALELLEAQSKFTKKELQILYRGFKNECPSGVVNEETFKEIYSQFFPQGDSTTYAHFLFNAFDTDHNGAVSFEDFIKGLSILLRGTVQEKLNWAFNLYDINKDGYITKEEMLDIMKAIYDMMGKCTYPVLKEDAPRQHVETFFQKMDKNKDGVVTIDEFIESCQKDENIMRSMQLFENVI Predict reactive species |
| Full Name | Kv channel interacting protein 4 |
| Calculated Molecular Weight | 250 aa, 28 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC032520 |
| Gene Symbol | KCNIP4 |
| Gene ID (NCBI) | 80333 |
| RRID | AB_2130271 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q6PIL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KCNIP4 is a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belong to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KCNIP4 antibody 60133-1-Ig | Download protocol |
| IHC protocol for KCNIP4 antibody 60133-1-Ig | Download protocol |
| WB protocol for KCNIP4 antibody 60133-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KCNIP4 Monoclonal antibody (5B1D12), catalog number 60133-1-Ig, has a total of 3 citations. These citations appear in Nature Communications, iScience, and bioRxiv. The research fields associated with these citations include Alzheimer's disease resilience in neocortical neurons and epileptic cognitive impairment, both falling within the broader neuroscience domain. The antibody was applied via WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Molecular signatures of resilience to Alzheimer's disease in neocortical layer 4 neurons. | ||
iScience Single-nucleus RNA sequencing-based construction of a hippocampal neuron atlas in mice with epileptic cognitive impairment | ||
bioRxiv Molecular Signatures of Resilience to Alzheimer's Disease in Neocortical Layer 4 Neurons |
