KCNJ1 Polyclonal antibody

KCNJ1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20953-1-AP

Synonyms

KCNJ1, KIR1.1, ROMK, ROMK1

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Validation Data Gallery

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  WB analysis of mouse brain using 20953-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20953-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20953-1-AP

  IP Result of anti-KCNJ1 (IP:20953-1-AP, 4ug; Detection:20953-1-AP 1:300) with mouse brain tissue lysate 3600ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 20953-1-AP is KCNJ1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCNJ1 fusion protein Ag15100
• Full Name: potassium inwardly-rectifying channel, subfamily J, member 1
• Calculated molecular weight: 391 aa, 45 kDa
• Observed molecular weight: 75-80 kDa
• GenBank accession number: BC074752
• Gene symbol: KCNJ1
• Gene ID (NCBI): 3758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
		Am J Physiol Renal Physiol; (Pro)Renin Receptor Regulates Potassium Homeostasis through a Local Mechanism.; Authors - Chuanming Xu
mouse	IF	J Am Soc Nephrol; Activation of mTORC1 in Collecting Ducts Causes Hyperkalemia.; Authors - Zhenguo Chen