Kir4.1 Polyclonal antibody

Kir4.1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12503-1-AP

Synonyms

BIRK 10, KCNJ10, KCNJ13 PEN, KIR1.2, Kir4.1



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12503-1-AP targets Kir4.1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kir4.1 fusion protein Ag3182
Full Name potassium inwardly-rectifying channel, subfamily J, member 10
Calculated molecular weight 379 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC034036
Gene symbol KCNJ10
Gene ID (NCBI) 3766
RRIDAB_2234144
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Kir4.1 antibody 12503-1-APDownload protocol
IHC protocol for Kir4.1 antibody 12503-1-APDownload protocol
IP protocol for Kir4.1 antibody 12503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Neuron

Glioma epileptiform activity and progression are driven by IGSF3-mediated potassium dysregulation

Authors - Rachel Naomi Curry

Glia

Wnt/β-catenin signaling mediates the seizure-facilitating effect of postischemic reactive astrocytes after pentylenetetrazole-kindling.

Authors - Jialei Yang
mouseIF

Glia

Elevated GFAP induces astrocyte dysfunction in caudal brain regions: A potential mechanism for hindbrain involved symptoms in type II Alexander disease.

Authors - Heather R Minkel
humanWB

Mult Scler

Low reliability of anti-KIR4.183-120 peptide auto-antibodies in multiple sclerosis patients.

Authors - Mariapaola Marino
mouse,rabbitWB

Exp Eye Res

A novel model of subretinal edema induced by DL-alpha aminoadipic acid

Authors - Zhan Xie
ratWB,IF

Am J Transl Res

Yigan Mingmu Decoction inhibits diabetic macular edema through regulating Kir4.1/AQP4 axis: a study based on network pharmacology

Authors - Longrong Huang