KCNJ14 Polyclonal antibody

KCNJ14 Polyclonal Antibody for WB, ELISA

Cat No. 14171-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

IRK 4, IRK4, KCNJ14, KIR2.4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis using 14171-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14171-1-AP (KCNJ14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A549 using 14171-1-AP
WB result of KCNJ14 antibody (14171-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KCNJ14 transfected A549 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

mouse brain tissue, A549 cells, rat brain tissue

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

14171-1-AP targets KCNJ14 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5370 Product name: Recombinant human KCNJ14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 182-436 aa of BC035918 Sequence: GAVMAKMAKPKKRNETLVFSENAVVALRDHRLCLMWRVGNLRRSHLVEAHVRAQLLQPRVTPEGEYIPLDHQDVDVGFDGGTDRIFLVSPITIVHEIDSASPLYELGRAELARADFELVVILEGMVEATAMTTQCRSSYLPGELLWGHRFEPVLFQRGSQYEVDYRHFHRTYEVPGTPVCSAKELDERAEQASHSLKSSFPGSLTAFCYENELALSCCQEEDEDDETEEGNGVETEDGAASPRVLTPTLALTLPP Predict reactive species
Full Name	potassium inwardly-rectifying channel, subfamily J, member 14
Calculated Molecular Weight	436 aa, 48 kDa
Observed Molecular Weight	48 kDa
GenBank Accession Number	BC035918
Gene Symbol	KCNJ14
Gene ID (NCBI)	3770
RRID	AB_2878024
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UNX9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for KCNJ14 antibody 14171-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	BMC Med Genomics KCNJ14 knockdown significantly inhibited the proliferation and migration of colorectal cells Authors - Bin Li View Article