Kir5.1 Polyclonal antibody

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  WB analysis using 12696-1-AP

  HEK-293 cells(heated at 100 ° C, heated at 37℃) and Hela cells(heated at 100 ° C, heated at 37℃) lysates were subjected to SDS PAGE followed by western blot with 12696-1-AP (Kir5.1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12696-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12696-1-AP (Kir5.1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Kir5.1 Polyclonal Antibody for WB, ELISA

Cat No. 12696-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KCNJ16, BIR9

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12696-1-AP targets Kir5.1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kir5.1 fusion protein Ag3405
• Full Name: potassium inwardly-rectifying channel, subfamily J, member 16
• Calculated Molecular Weight: 418 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC033038
• Gene Symbol: Kir5.1
• Gene ID (NCBI): 3773
• RRID: AB_2130617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NPI9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kir5.1, also known as KCNJ16, is a member of the inwardly rectifying potassium (Kir) channel family, which plays crucial roles in various physiological processes. These channels are characterized by their ability to allow potassium ions to flow more freely into the cell than out, a property that is vital for maintaining the resting membrane potential and regulating excitability in cardiac, neuronal, and smooth muscle tissues. Kir5.1 is specifically noted for its unique expression patterns and functional roles in several tissues, particularly in the kidney and brain.