KCNJ3 Polyclonal antibody

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  WB analysis of mouse brain using 16985-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16985-1-AP (KCNJ3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16985-1-AP

  Immunofluorescent analysis of HeLa cells using 16985-1-AP (KCNJ3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

KCNJ3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16985-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

G protein-activated inward rectifier potassium channel 1, GIRK 1, GIRK1, GIRK-1, KGA

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16985-1-AP targets KCNJ3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10581

  Product name: Recombinant human KCNJ3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 147-501 aa of BC022495

  Sequence: GYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTIRHVIDAKSPFYDLSQRSMQTKQFEIVVILEGIVETTGMTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFEVPTPPYSVKEQEEMLLMSSPLIAPAITNSKERHNSVECLDGLDDITTKLPSKLQKITGREDFPKKLLRMSSTTSEKAYSLGDLPMKLQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGAARMEGNLPAKLRKMNSDRFT

• Full Name: potassium inwardly-rectifying channel, subfamily J, member 3
• Calculated Molecular Weight: 501 aa, 57 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC022495
• Gene Symbol: KCNJ3
• Gene ID (NCBI): 3760
• RRID: AB_2878336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48549
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.