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Kir6.1 Polyclonal antibody

Kir6.1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14954-1-AP

Synonyms

KCNJ8, Kir6.1, uKATP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using Kir6.1 Polyclonal antibody (14954-1-AP)

    WB analysis of mouse spleen using 14954-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14954-1-AP (Kir6.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using Kir6.1 Polyclonal antibody (14954-1-AP)

    IP experiment of mouse spleen using 14954-1-AP

    IP Result of anti-Kir6.1 (IP:14954-1-AP, 4ug; Detection:14954-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

Tested Applications

Positive WB detected inmouse spleen tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

14954-1-AP targets Kir6.1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kir6.1 fusion protein Ag6792
Full Name potassium inwardly-rectifying channel, subfamily J, member 8
Calculated molecular weight 48 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC000544
Gene symbol KCNJ8
Gene ID (NCBI) 3764
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Kir6.1 antibody 14954-1-APDownload protocol
IP protocol for Kir6.1 antibody 14954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Pharmacol Res Perspect

Tetramisole is a new IK1 channel agonist and exerts IK1 -dependent cardioprotective effects in rats.

Authors - Qinghua Liu
View Article
mouseWB,IF

Brain Behav Immun

Opening K(ATP) channels induces inflammatory tolerance and prevents chronic pain

Authors - Cheng Qian
View Article