KCNK4 Polyclonal antibody

KCNK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27113-1-AP

Synonyms

K2p4.1, KCNK4, TRAAK, TRAAK1, Two pore K(+) channel KT4.1

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Validation Data Gallery

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  WB analysis using 27113-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27113-1-AP (KCNK4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 27113-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 27113-1-AP (KCNK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 27113-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27113-1-AP (KCNK4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 27113-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27113-1-AP (KCNK4 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse eye tissue, rat eye tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 27113-1-AP is KCNK4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCNK4 fusion protein Ag25931
• Full Name: potassium channel, subfamily K, member 4
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC033577
• Gene Symbol: KCNK4
• Gene ID (NCBI): 50801
• RRID: AB_2880761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Funct Integr Genomics; LncRNA SLC25A21-AS1 increases the chemosensitivity and inhibits the progression of ovarian cancer by upregulating the expression of KCNK4; Authors - Ke Huang