Tested Applications
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18033-1-AP targets KCNK9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12603 Product name: Recombinant human KCNK9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 246-374 aa of BC075079 Sequence: FLTMNSEDERRDAEERASLAGNRNSMVIHIPEEPRPSRPRYKADVPDLQSVCSCTCYRSQDYGGRSVAPQNSFSAKLAPHYFHSISYKIEEISPSTLKNSLFPSPISSISPGLHSFTDHQRLMKRRKSV Predict reactive species |
| Full Name | potassium channel, subfamily K, member 9 |
| Calculated Molecular Weight | 374 aa, 42 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC075079 |
| Gene Symbol | KCNK9 |
| Gene ID (NCBI) | 51305 |
| RRID | AB_3741942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPC2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNK9 antibody 18033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
This product has received 2 reviews, both rated 4 out of 5 stars. One review covers Western Blot at a 1:2000 dilution, and the other covers Immunofluorescence at a 1:500 dilution, both performed on Primary Embryonic Cortical Neurons (DIV10). No written comments were provided, but the accompanying images demonstrate clear and specific signal for both applications. Overall, the reviews suggest reliable performance for detecting the target in neuronal cell preparations.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Connor (Verified Customer) (08-24-2018) |
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Connor (Verified Customer) (08-24-2018) |
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