KCNMB4 Monoclonal antibody

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• KCNMB4 Monoclonal antibody (60122-1-Ig)

KCNMB4 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 60122-1-Ig Copied!

Clone No. 4H7G3

Publications (5)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

4H7G3, BK channel subunit beta 4, BK channel subunit beta-4, BKbeta4, Calcium-activated potassium channel subunit beta-4

• 

  WB analysis of fetal human brain using 60122-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 60122-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60122-1-Ig (KCNMB4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: fetal human brain tissue, human brain tissue
• Positive IHC detected in: human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60122-1-Ig targets KCNMB4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5618

  Product name: Recombinant human KCNMB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 46-176 aa of BC042446

  Sequence: DLQATEANCTVLSVQQIGEVFECTFTCGADCRGTSQYPCVQVYVNNSESNSRALLHSDEHQLLTNPKCSYIPPCKRENQKNLESVMNWQQYWKDEIGSQPFTCYFNQHQRPDDVLLHRTHDEIVLLHCFLW

• Full Name: potassium large conductance calcium-activated channel, subfamily M, beta member 4
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC042446
• Gene Symbol: KCNMB4
• Gene ID (NCBI): 27345
• RRID: AB_2280832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q86W47
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The KCNMB4 Monoclonal antibody (4H7G3), catalog number 60122-1-Ig, has a total of 5 citations. These appear in Cell Death Dis, Mitochondrion, Biomed Pharmacother, FASEB J, and Peptides. The associated research fields span nasopharyngeal carcinoma and paclitaxel resistance, bronchial epithelium physiology, mitochondrial BKCa channel signaling in endothelial cells, endothelial-to-mesenchymal transition in diabetic nephropathy, and renal hemodynamics under diabetic conditions.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Cell Death Dis; PRMT5 upregulates KCNMB4 expression via histone methylation to promote paclitaxel resistance in advanced nasopharyngeal carcinoma.; Authors - Lizhen Liu
human	WB	Mitochondrion; Deficiency of the BKCa potassium channel displayed significant implications for the physiology of the human bronchial epithelium; Authors - Kamila Maliszewska-Olejniczak
human	WB	Biomed Pharmacother; Cytoprotective effects of the flavonoid quercetin by activating mitochondrial BKCa channels in endothelial cells.; Authors - Rafał Paweł Kampa
		FASEB J; Apelin Alleviated Endothelial-To-Mesenchymal Transition via Increasing BKCa in Diabetic Nephropathy; Authors - Chunmeng Fan
		Peptides; Apelin/APJ increased renal blood flow through endothelial BKCa channel induced p-eNOS and ET-1 in diabetic conditions; Authors - Mingcong Huang