Tested Applications
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17188-1-AP targets KCNN3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10972 Product name: Recombinant human KCNN3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 550-736 aa of BC042147 Sequence: KLELTKAEKHVHNFMMDTQLTKRIKNAAANVLRETWLIYKHTKLLKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLSDQANTLVDLSKMQNVMYDLITELNDRSEDLEKQIGSLESKLEHLTASFNSLPLLIADTLRQQQQQLLSAIIEARGVSVAVGTTHTPISDSPIGVSSTSFPTPYTSSSSC Predict reactive species |
| Full Name | potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3 |
| Calculated Molecular Weight | 426aa,48 kDa; 736aa,82 kDa |
| GenBank Accession Number | BC042147 |
| Gene Symbol | KCNN3 |
| Gene ID (NCBI) | 3782 |
| RRID | AB_2878358 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UGI6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KCNN3 antibody 17188-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KCNN3 Polyclonal antibody (Cat# 17188-1-AP) has 2 citations. One appears in Frontiers in Cellular Neuroscience, studying potassium channel conductance in serotonergic neurons of the dorsal raphe nucleus (neuroscience). The other is published in Tissue and Cell, investigating how the P2X7 receptor regulates SK3 channels to promote gastric cancer progression (oncology). Applications cited include IF and WB, with species covering mouse and human.
| Species | Application | Title |
|---|---|---|
Front Cell Neurosci Potassium Channel Conductance Is Involved in Phenylephrine-Induced Spontaneous Firing of Serotonergic Neurons in the Dorsal Raphe Nucleus. | ||
Tissue Cell Purinergic ion channel-type P2X7 receptor regulates SK3 channel to promote the progression of gastric cancer. |
