KCNN4 Polyclonal antibody

• 

  WB analysis of HEK-293 using 23271-1-AP

  WB result of KCNN4 antibody (23271-1-AP, 1:4000) with si-control and si-KCNN4 transfected HEK293 cells.

• 

  WB analysis using 23271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23271-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 23271-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KCNN4 antibody (23271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

KCNN4 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 23271-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

KCa3.1, Intermediate conductance calcium-activated potassium channel protein 4, IKCA1, IK1, hSK4

---

Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HEK-293 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

23271-1-AP targets KCNN4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19774

  Product name: Recombinant human KCNN4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 128-427 aa of BC015337

  Sequence: VQDLGAPLTSPQPWPGFLGQGEALLSLAMLLRLYLVPRAVLLRSGVLLNASYRSIGALNQVRFRHWFVAKLYMNTHPGRLLLGLTLGLWLTTAWVLSVAERQAVNATGHLSDTLWLIPITFLTIGYGDVVPGTMWGKIVCLCTGVMGVCCTALLVAVVARKLEFNKAEKHVHNFMMDIQYTKEMKESAARVLQEAWMFYKHTRRKESHAARRHQRKLLAAINAFRQVRLKHRKLREQVNSMVDISKMHMILYDLQQNLSSSHRALEKQIDTLAGKLDALTELLSTALGPRQLPEPSQQSK

• Full Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
• Calculated Molecular Weight: 427 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC015337
• Gene Symbol: KCNN4
• Gene ID (NCBI): 3783
• RRID: AB_2879243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: O15554
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cancer Cell Int; KCNN4 may weaken anti-tumor immune response via raising Tregs and diminishing resting mast cells in clear cell renal cell carcinoma.; Authors - Yankang Cui
human	WB,IHC,FC	Cancer Biomark; KCNN4 promotes the progression of lung adenocarcinoma by activating the AKT and ERK signaling pathways; Authors - Ping Xu ; KD Validated
human	WB	Front Physiol; New KCNN4 Variants Associated With Anemia: Stomatocytosis Without Erythrocyte Dehydration; Authors - B. Allegrini
mouse,canine	WB,IHC,IF	Cardiovasc Ther; KCa3.1 Promotes Proinflammatory Exosome Secretion by Activating AKT/Rab27a in Atrial Myocytes during Rapid Pacing; Authors - Dishiwen Liu
human	IHC	Biochem Biophys Res Commun; Integrated expression profiling of potassium channels identifys KCNN4 as a prognostic biomarker of pancreatic cancer.; Authors - Shuheng Jiang
mouse	WB	Gels; Comprehensive Optimization of Western Blotting; Authors - Dishiwen Liu