KCNN4 Polyclonal antibody

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• KCNN4 Polyclonal antibody (23271-1-AP)

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• KD/KO Validated

KCNN4 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 23271-1-AP Copied!

Publications (15)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

KCa3.1, hIKCa1, hKCa4, hSK4, IK1

• 

  WB analysis using 23271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 23271-1-AP

  WB result of KCNN4 antibody (23271-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KCNN4 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 23271-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 23271-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KCNN4 antibody (23271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, MCF-7 cells, HEK-293 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23271-1-AP targets KCNN4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19774

  Product name: Recombinant human KCNN4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 128-427 aa of BC015337

  Sequence: VQDLGAPLTSPQPWPGFLGQGEALLSLAMLLRLYLVPRAVLLRSGVLLNASYRSIGALNQVRFRHWFVAKLYMNTHPGRLLLGLTLGLWLTTAWVLSVAERQAVNATGHLSDTLWLIPITFLTIGYGDVVPGTMWGKIVCLCTGVMGVCCTALLVAVVARKLEFNKAEKHVHNFMMDIQYTKEMKESAARVLQEAWMFYKHTRRKESHAARRHQRKLLAAINAFRQVRLKHRKLREQVNSMVDISKMHMILYDLQQNLSSSHRALEKQIDTLAGKLDALTELLSTALGPRQLPEPSQQSK

• Full Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
• Calculated Molecular Weight: 427 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC015337
• Gene Symbol: KCNN4
• Gene ID (NCBI): 3783
• RRID: AB_2879243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: O15554
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).

Publications

What published studies show

The anti-KCNN4 antibody (Cat# 23271-1-AP) has 15 total citations. These appear in journals including Nature Communications, Journal of Hematology & Oncology, Journal of Biological Chemistry, European Journal of Pharmacology, International Journal of Molecular Sciences, Experimental Cell Research, and others. Research fields span pancreatic cancer, prostate cancer, lung adenocarcinoma, renal cell carcinoma, rheumatoid arthritis, ion channel biology, macrophage polarization, cardiac electrophysiology, and anemia. Applications include WB, IHC, IF, IP, and CoIP in human, mouse, and canine samples.

Automatically generated from the 15 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	J Hematol Oncol; The LINC00623/NAT10 signaling axis promotes pancreatic cancer progression by remodeling ac4C modification of mRNA; Authors - Zengyu Feng
human	WB	Nat Commun; Phospholipid scrambling induced by an ion channel/metabolite transporter complex; Authors - Han Niu
human	IF	Cancer Cell Int; KCNN4 may weaken anti-tumor immune response via raising Tregs and diminishing resting mast cells in clear cell renal cell carcinoma.; Authors - Yankang Cui
mouse	WB	Gels; Comprehensive Optimization of Western Blotting; Authors - Dishiwen Liu
mouse	WB,IF	Eur J Pharmacol; Blockade of KCa3.1 ameliorates rheumatoid arthritis by suppressing macrophage M1 polarization via the NLRP3 inflammasome signaling pathway.; Authors - Jing Xing ; KO Validated
human	WB	Int J Mol Sci; Downregulation of IL-8 and IL-10 by the Activation of Ca2+-Activated K+ Channel KCa3.1 in THP-1-Derived M2 Macrophages; Authors - Susumu Ohya