KCTD12 Polyclonal antibody

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• KD/KO Validated

KCTD12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15523-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BTB/POZ domain-containing protein KCTD12, C13orf2, PFET1, Pfetin

• 

  WB analysis of HEK-293 using 15523-1-AP

  WB result of KCTD12 antibody (15523-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-KCTD12 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 15523-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 15523-1-AP

  Hela cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 15523-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 15523-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 15523-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 15523-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 15523-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 15523-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 15523-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 15523-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 15523-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 15523-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KCTD12 antibody (15523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 15523-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using KCTD12 antibody (15523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, HeLa cells, human brain tissue, MCF-7 cells, rat brain tissue
• Positive IHC detected in: human bowen disease, human intrahepatic cholangiocarcinoma tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15523-1-AP targets KCTD12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5716

  Product name: Recombinant human KCTD12 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-325 aa of BC013764

  Sequence: MALADSTRGLPNGGGGGGGSGSSSSSAEPPLFPDIVELNVGGQVYVTRRCTVVSVPDSLLWRMFTQQQPQELARDSKGRFFLDRDGFLFRYILDYLRDLQLVLPDYFPERSRLQREAEYFELPELVRRLGAPQQPGPGPPPSRRGVHKEGSLGDELLPLGYSEPEQQEGASAGAPSPTLELASRSPSGGAAGPLLTPSQSLDGSRRSGYITIGYRGSYTIGRDAQADAKFRRVARITVCGKTSLAKEVFGDTLNESRDPDRPPERYTSRYYLKFNFLEQAFDKLSESGFHMVACSSTGTCAFASSTDQSEDKIWTSYTEYVFCRE

• Full Name: potassium channel tetramerisation domain containing 12
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC013764
• Gene Symbol: KCTD12
• Gene ID (NCBI): 115207
• RRID: AB_2132006
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CX2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCTD12 also known as PFET1 or PFETIN, which contains a voltage-gated potassium (K+) channel tetramerization T1 domain and a BTB/POZ (Bric-a-brac, Tram-track, Broad complex poxvirus and zinc finger) domain, belongs to the KCTD family and was initially identified in cochlea. In addition to being a K+ channel protein that responds to membrane potential7, KCTD12 also acts as an auxiliary subunit of GABAB (γ-aminobutyric acid type B) receptors, which regulate emotionality and neuronal excitability.

Publications

What published studies show

The KCTD12 antibody (Cat# 15523-1-AP) has 10 citations published in Nature Communications, Advanced Science, Oncogene, Cell Death & Disease, Pharmacological Research, Scientific Reports, Journal of Neuroscience, Oncology Reports, Frontiers in Molecular Neuroscience, and PLoS ONE. Research fields span neuroscience (GABA receptor function, ALS, synaptic plasticity), oncology (colorectal cancer, uveal melanoma, ferroptosis), cell cycle regulation, and fibrosis.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Commun; Purkinje cell microzones mediate distinct kinematics of a single movement; Authors - François G C Blot
		Adv Sci (Weinh); BIN1 and ALDH1B1 Deficiency in Colonic Smooth Muscle Drives Mitochondrial Dysfunction and Fibrosis in Slow-Transit Constipation.; Authors - Jianbo Liu
mouse	WB,IF	Pharmacol Res; The effects of Kctd12, an auxiliary subunit of GABAB receptor in dentate gyrus on behavioral response to chronic social defeat stress in mice.; Authors - Si-Long Deng
human	WB	Cell Death Dis; Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axis; Authors - Lifu Luo
human	WB,IHC,IF	Oncogene; KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition.; Authors - Y Zhong ; KD Validated
human	WB,IHC	Sci Rep; KCTD12 Regulates Colorectal Cancer Cell Stemness through the ERK Pathway.; Authors - Liping Li ; KD Validated