Tested Applications
| Positive IHC detected in | human stomach cancer tissue, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21111-1-AP targets KCTD14 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14265 Product name: Recombinant human KCTD14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-225 aa of BC001929 Sequence: MSTVVELNVGGEFHTTTLGTLRKFPGSKLAEMFSSLAKASTDAEGRFFIDRPSTYFRPILDYLRTGQVPTQHIPEVYREAQFYEIKPLVKLLEDMPQIFGEQVSRKQFLLQVPGYSENLELMVRLARAEAITARKSSVLVCLVETEEQDAYYSEVLCFLQDKKMFKSVVKFGPWKAVLDNSDLMHCLEMDIKAQGYKVFSKFYLTYPTKRNEFHFNIYSFTFTWW Predict reactive species |
| Full Name | potassium channel tetramerisation domain containing 14 |
| Calculated Molecular Weight | 225 aa, 26 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC001929 |
| Gene Symbol | KCTD14 |
| Gene ID (NCBI) | 65987 |
| RRID | AB_10733343 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQ13 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KCTD14, or potassium channel tetramerization domain containing 14, is a member of the KCTD family of proteins. This family consists of 25 members in humans, many of which are only partially characterized . KCTD14, like other members of the KCTD family, contains a conserved domain known as the BTB (Broad complex, Tramtrak, and Bric-a-brac)/POZ (poxvirus zinc finger) domain, which is crucial for protein oligomerization and establishing protein-protein interactions. KCTD14 has been implicated in various biological functions, and recent research suggests a potential role in cancer. Although not as extensively studied as some other KCTD members, KCTD14 has been noted in database analyses to potentially play a protumor role in ovarian cancer. Specifically, the COSMIC database reports a copy number variation (CNV) gain in 4.5% of ovarian cancers, and the GENT2 database indicates a fold change (FC) of 1.5 in expression, with a p-value less than 0.001, suggesting a possible association with ovarian cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KCTD14 antibody 21111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KCTD14 Polyclonal antibody (catalog number 21111-1-AP) has a total of 1 citation. This citation appears in the journal iScience, published in 2026. The associated research field is neuroscience, specifically examining the role of olfactory receptor Olfr544 in neuronal cells, with insights into neurite outgrowth and CREB signaling. The antibody is a rabbit IgG polyclonal reactive with human and mouse, applicable for WB, IHC, and ELISA.
| Species | Application | Title |
|---|---|---|
iScience Role of olfactory receptor Olfr544 in neuronal cells: Insights into neurite outgrowth and CREB signaling. |
