KDM5B Polyclonal antibody

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  WB analysis using 28346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28346-1-AP (KDM5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

KDM5B Polyclonal Antibody for WB, ELISA

Cat No. 28346-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Lysine specific demethylase 5B, lysine (K) demethylase 5B, JARID1B, Histone demethylase JARID1B, CT31

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Tested Applications

• Positive WB detected in: A375 cells, HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28346-1-AP targets KDM5B in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28821

  Product name: Recombinant human KDM5B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 190-309 aa of NM_001314042

  Sequence: SLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDL

• Full Name: lysine (K)-specific demethylase 5B
• Calculated Molecular Weight: 176 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: NM_001314042
• Gene Symbol: KDM5B
• Gene ID (NCBI): 10765
• RRID: AB_3669654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UGL1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.