Tested Applications
| Positive WB detected in | TF-1 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22739-1-AP targets KDM5D in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18690 Product name: Recombinant human KDM5D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1375-1482 aa of BC146767 Sequence: SRTRSRALERRRRRQKVDQGRNVENLVQQELQSKRARSSGIMSQVGREEEHYQEKADRENMFLTPSTDHSPFLKGNQNSLQHKDSGSSAACPSLMPLLQLSYSDEQQL Predict reactive species |
| Full Name | lysine (K)-specific demethylase 5D |
| Calculated Molecular Weight | 1482 aa, 167 kDa |
| Observed Molecular Weight | 70 kDa, 175 kDa |
| GenBank Accession Number | BC146767 |
| Gene Symbol | KDM5D |
| Gene ID (NCBI) | 8284 |
| RRID | AB_2879160 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BY66 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KDM5D antibody 22739-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KDM5D Polyclonal antibody (Cat# 22739-1-AP) has 4 citations published in Nature Metabolism, International Journal of Biological Sciences, Cell Signaling, and Translational Andrology and Urology. The research fields span epigenetic regulation of gene expression, tumorigenesis, chemoresistance mechanisms, colorectal cancer, and prostate cancer treatment response prediction.
| Species | Application | Title |
|---|---|---|
Nat Metab Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis. | ||
Cell Signal KDM5D epigenetically represses FERD3L to inhibit tumor growth in male colorectal cancer. | ||
Transl Androl Urol KDM5D predicts response to docetaxel chemotherapy in metastatic castration resistant prostate cancer patients. |

