KDM5D Polyclonal antibody

KDM5D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22739-1-AP

Synonyms

H Y, Histocompatibility Y antigen, Histone demethylase JARID1D, HY, HYA, JARID1D, KDM5D, KIAA0234, lysine (K) demethylase 5D, Lysine specific demethylase 5D, Protein SmcY, SMCY

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Validation Data Gallery

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  WB analysis using 22739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22739-1-AP (KDM5D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: TF-1 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

22739-1-AP targets KDM5D in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KDM5D fusion protein Ag18690
• Full Name: lysine (K)-specific demethylase 5D
• Calculated Molecular Weight: 1482 aa, 167 kDa
• Observed Molecular Weight: 70 kDa, 175 kDa
• GenBank Accession Number: BC146767
• Gene Symbol: KDM5D
• Gene ID (NCBI): 8284
• RRID: AB_2879160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; KDM5B demethylates H3K4 to recruit XRCC1 and promote chemoresistance.; Authors - Wenxia Xu
human	IHC	Transl Androl Urol; KDM5D predicts response to docetaxel chemotherapy in metastatic castration resistant prostate cancer patients.; Authors - Georg Schäfer
human	WB	Nat Metab; Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.; Authors - Shi-Ting Li