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KDM5D Polyclonal antibody

KDM5D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

H Y, Histocompatibility Y antigen, Histone demethylase JARID1D, HY, HYA, JARID1D, KDM5D, KIAA0234, lysine (K) demethylase 5D, Lysine specific demethylase 5D, Protein SmcY, SMCY

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inTF-1 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22739-1-AP targets KDM5D in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18690

Product name: Recombinant human KDM5D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1375-1482 aa of BC146767

Sequence: SRTRSRALERRRRRQKVDQGRNVENLVQQELQSKRARSSGIMSQVGREEEHYQEKADRENMFLTPSTDHSPFLKGNQNSLQHKDSGSSAACPSLMPLLQLSYSDEQQL

Predict reactive species
Full Name lysine (K)-specific demethylase 5D
Calculated Molecular Weight 1482 aa, 167 kDa
Observed Molecular Weight70 kDa, 175 kDa
GenBank Accession NumberBC146767
Gene Symbol KDM5D
Gene ID (NCBI) 8284
RRIDAB_2879160
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY66
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KDM5D antibody 22739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The KDM5D Polyclonal antibody (Cat# 22739-1-AP) has 4 citations published in Nature Metabolism, International Journal of Biological Sciences, Cell Signaling, and Translational Andrology and Urology. The research fields span epigenetic regulation of gene expression, tumorigenesis, chemoresistance mechanisms, colorectal cancer, and prostate cancer treatment response prediction.

SpeciesApplicationTitle
humanWB

Nat Metab

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Authors - Shi-Ting Li
humanWB

Int J Biol Sci

KDM5B demethylates H3K4 to recruit XRCC1 and promote chemoresistance.

Authors - Wenxia Xu

Cell Signal

KDM5D epigenetically represses FERD3L to inhibit tumor growth in male colorectal cancer.

Authors - Jianwei Yu
humanIHC

Transl Androl Urol

KDM5D predicts response to docetaxel chemotherapy in metastatic castration resistant prostate cancer patients.

Authors - Georg Schäfer
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