Tested Applications
| Positive WB detected in | mouse brain tissue, BGC-823 cells, A549 cells, human brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13563-1-AP targets KHDRBS3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4438 Product name: Recombinant human KHDRBS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-346 aa of BC032606 Sequence: MEEKYLPELMAEKDSLDPSFTHALRLVNQEIEKFQKGEGKDEEKYIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFLIPDYNDEIRQAQLQELTYLNGGSENADVPVVRGKPTLRTRGVPAPAITRGRGGVTARPVGVVVPRGTPTPRGVLSTRGPVSRGRGLLTPRARGVPPTGYRPPPPPPTQETYGEYDYDDGYGTAYDEQSYDSYDNSYSTPAQSGADYYDYGHGLSEETYDSYGQEEWTNSRHKAPSARTAKGVYRDQPYGRY Predict reactive species |
| Full Name | KH domain containing, RNA binding, signal transduction associated 3 |
| Calculated Molecular Weight | 346 aa, 38, 8 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC032606 |
| Gene Symbol | KHDRBS3 |
| Gene ID (NCBI) | 10656 |
| RRID | AB_2130319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75525 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KH domain-containing, RNA-binding, signal transduction-associated protein 3 (KHDRBS3, SLM2) is a RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion (15901763). May play a role as a negative regulator of cell growth. Inhibits cell proliferation. Involved in splice site selection of vascular endothelial growth factor. Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. RNA-binding abilities are down-regulated by tyrosine kinase PTK6. Involved in post-transcriptional regulation of HIV-1 gene expression (11741900).Ubiquitous with higher expression in testis, skeletal muscle and brain. Expressed in the kidney only in podocytes, the glomerular epithelial cells of the kidney. Strongly expressed after meiosis.This antibody is a rabbit polyclonal antibody raised against a full-length human KHDRBS3 protein.The observed molecular weight of KHDRBS is about 55kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KHDRBS3 antibody 13563-1-AP | Download protocol |
| WB protocol for KHDRBS3 antibody 13563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KHDRBS3 Polyclonal antibody (Cat# 13563-1-AP) has 6 total citations. These appear in Cell Death Dis, Acta Neuropathol, Int J Nanomedicine, Life Sci, PLoS Genet, and Am J Reprod Immunol. Research fields span blood–tumor barrier regulation, neurodegenerative diseases (FTLD/ALS), hepatocellular carcinoma, glioblastoma, tissue-specific RNA splicing, and ovarian cancer ferroptosis. Applications cited include WB, IHC, IF, and CoIP, with reactivity in human and mouse.
| Species | Application | Title |
|---|---|---|
Cell Death Dis KHDRBS3 regulates the permeability of blood-tumor barrier via cDENND4C/miR-577 axis.
| ||
Acta Neuropathol Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations. | ||
Int J Nanomedicine Exosome-Delivered circFOXP1 Upregulates Autophagy and Promotes Hepatocellular Carcinoma Progression Through Its Encoded p196 Protein Targeting the KHDRBS3/ULK1 Axis | ||
Life Sci KHDRBS3 facilitates self-renewal and temozolomide resistance of glioblastoma cell lines | ||
PLoS Genet The tissue-specific RNA binding protein T-STAR controls regional splicing patterns of neurexin pre-mRNAs in the brain. | ||
Am J Reprod Immunol Long non-coding RNA TPT1-AS1 inhibits ferroptosis in ovarian cancer by regulating GPX4 via CREB1 regulation |
Reviews
What customers say
All four reviews are for Western Blot applications. Three reviewers rated the antibody 5 stars, reporting strong, clear target bands in HEK293T, RKO, and mESC cells at dilutions of 1:1000–1:2000, with minimal non-specific binding. One reviewer gave 2 stars, noting that while the antibody produced a strong ~55 kDa band in mESC lysates, it was so sensitive that even a 1:10,000 dilution yielded a robust signal, suggesting the recommended dilution may be too concentrated for their setup. Overall, users find the antibody highly specific and sensitive for Khdrbs3 detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Moritz (Verified Customer) (09-26-2025) | Worked good - a strong band of the protein is visible (and few weaker non-specific ones)
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Aleksandr (Verified Customer) (11-10-2024) | Antibodies were used to detect Khdrbs3 in human RKO cells
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Tsimafei (Verified Customer) (08-07-2024) | Great antibody
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Julia (Verified Customer) (08-07-2024) | Antibody used for 30ug of mESC protein extract. There are few bands and strong signal at ~55kDa so it can be diluted even more than 1:10000 for similar set-up.
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