KHK Recombinant monoclonal antibody, PBS Only

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• KHK Recombinant monoclonal antibody, PBS Only (85386-1-PBS)

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  WB analysis using 85386-1-RR (same clone as 85386-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85386-1-RR (KHK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85386-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 85386-1-RR (same clone as 85386-1-PBS)

  WB result of KHK antibody (85386-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KHK transfected HepG2 cells. This data was developed using the same antibody clone with 85386-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85386-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85386-1-RR against Human KHK were performed. The affinity constant is 8.46 nM.

KHK Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85386-1-PBS

Clone No.242745D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

242745D5, EC:2.7.1.3, Ketohexokinase

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Product Information

85386-1-PBS targets KHK in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8182

  Product name: Recombinant human KHK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-298 aa of BC006233

  Sequence: MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTILSLLGAPCAFMGSMAPGHVADFVLDDLRRYSVDLRYTVFQTTGSVPIATVIINEASGSRTILYYDRSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV

• Full Name: ketohexokinase (fructokinase)
• Calculated Molecular Weight: 298 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC006233
• Gene Symbol: KHK
• Gene ID (NCBI): 3795
• RRID: AB_3744181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50053
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ketohexokinase catalyzes conversion of fructose to fructose-1-phosphate, it is the first enzyme with a specialized pathway that catabolizes dietary fructose. Fructose metabolism seems to provide cancer cells with the supplementary fuel required for proliferation and metastasis.