KIAA0090 Polyclonal antibody

KIAA0090 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26017-1-AP

Synonyms

EMC1, ER membrane protein complex subunit 1, KIAA0090

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Validation Data Gallery

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  WB analysis of HeLa using 26017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26017-1-AP (KIAA0090 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26017-1-AP (KIAA0090 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26017-1-AP (KIAA0090 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 26017-1-AP is KIAA0090 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIAA0090 fusion protein Ag23406
• Full Name: KIAA0090
• Observed Molecular Weight: 112 kDa
• GenBank Accession Number: BC034589
• Gene Symbol: KIAA0090
• Gene ID (NCBI): 23065
• RRID: AB_2880337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	FEBS J; Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice; Authors - Xiao Li