KIAA0090 Polyclonal antibody

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• KIAA0090 Polyclonal antibody (26017-1-AP)

KIAA0090 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26017-1-AP Copied!

Publications (2)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

EMC1, PSEC0263

• 

  WB analysis of HeLa using 26017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26017-1-AP (KIAA0090 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26017-1-AP (KIAA0090 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse epididymis using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat epididymis using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded rat epididymis tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26017-1-AP (KIAA0090 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 26017-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26017-1-AP (KIAA0090 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, mouse colon tissue, rat colon tissue, rat epididymis tissue, human placenta tissue, human liver cancer tissue, mouse epididymis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26017-1-AP targets KIAA0090 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23406

  Product name: Recombinant human KIAA0090 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 439-788 aa of BC034589

  Sequence: KVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSE

• Full Name: KIAA0090
• Observed Molecular Weight: 112 kDa
• GenBank Accession Number: BC034589
• Gene Symbol: KIAA0090
• Gene ID (NCBI): 23065
• RRID: AB_2880337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N766
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The KIAA0090 Polyclonal antibody (Cat# 26017-1-AP) has 2 citations. They appear in FEBS Journal and bioRxiv. The cited research covers retinal degeneration caused by EMC1 deletion in photoreceptor cells (mouse, WB/IF) and the role of the ER membrane protein complex as a chaperone promoting biogenesis of multi-bundle membrane proteins (human, WB).

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	FEBS J; Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice; Authors - Xiao Li
human	WB	bioRxiv; The ER membrane protein complex acts as a chaperone to promote the biogenesis of multi-bundle membrane proteins.; Authors - Marinda Stanton