KIAA0182 Polyclonal antibody

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  WB analysis using 24947-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24947-1-AP (KIAA0182 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 24947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24947-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24947-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 24947-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KIAA0182 antibody (24947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

KIAA0182 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24947-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Genetic suppressor element 1, GSE1, KIAA0182

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: mouse testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Product Information

24947-1-AP targets KIAA0182 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20674

  Product name: Recombinant human KIAA0182 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 764-1113 aa of BC037556

  Sequence: TIFNLTHISAEKRKDKERLVEMLRAMKQKALSAAVADSLTNSPRDSPAVSLSEPATQQASLDVEKPVGAAASLSDIPKAAEPGKLEQVRPQELSRVQELAPASGEKARLSEAPGGKKSLSMLHYIRGAAPKDIPVPLSHSTNGKSKPWEPFVAEEFAHQFHESVLQSTQKALQKHKGSVAVLSAEQNHKVDTSVHYNIPELQSSSRAPPPQHNGQQEPPTARKGPPTQELDRDSEEEEEEDDEDGEDEEEVPKRKWQGIEAVFEAYQEHIEEQNLERQVLQTQCRRLEARHYSLSLTAEQLSHSVAELRSQKQKMVSERERLQAELDHLRKCLALPAMHWPRGYLKGYPR

• Full Name: KIAA0182
• Calculated Molecular Weight: 1217 aa, 136 kDa
• Observed Molecular Weight: 136 kDa
• GenBank Accession Number: BC037556
• Gene Symbol: KIAA0182
• Gene ID (NCBI): 23199
• RRID: AB_2879815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14687
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Cell Stem Cell; Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis.; Authors - Miller Huang ; KO Validated
human	WB	Nat Commun; The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs; Authors - Andreas Herchenröther
human	WB	Sci Adv; Targeting the scaffolding role of LSD1 (KDM1A) poises acute myeloid leukemia cells for retinoic acid-induced differentiation.; Authors - Roberto Ravasio
human	WB	EMBO Mol Med; LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.; Authors - Lukas Leiendecker
human	WB,IHC	J Biol Chem; GSE1 predicts poor survival outcome in gastric cancer patients by SLC7A5 enhancement of tumor growth and metastasis.; Authors - Keshuo Ding ; KD Validated
human	WB	Biochem Biophys Res Commun; GSE1 negative regulation by miR-489-5p promotes breast cancer cell proliferation and invasion.; Authors - Peng Chai