Rubicon Antibody

Name: Rubicon Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 21444-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 21444-1-AP

Synonyms

Baron, KIAA0226, RUBCN, Rubicon

WB analysis of mouse liver tissue using 21444-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 21444-1-AP(Rubicon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours
IHC staining of human testis tissue using 21444-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP(Rubicon antibody) at dilution of 1:50 (under 10x lens)
IHC staining of human testis tissue using 21444-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP(Rubicon antibody) at dilution of 1:50 (under 40x lens)

Cat No. 21444-1-AP

Freight/Packing: -

Country/Region

Size/Concentration

Quantity

Please visit your regions distributor:

Scandinavian customers can now order directly from Proteintech. Alternatively, you can also order through our approvied distributors Nordic Biosite.

Positive WB detected in	mouse liver tissue, A549 cells, HeLa cells
Positive IHC detected in	human testis tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

The 21444-1-AP immunogen is Fusion Protein Rubicon protein expressed in E. coli.

Reactivity	human, mouse, rat
Cited Species	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Rubicon fusion protein Ag13715
Full Name	KIAA0226
Calculated molecular weight	972aa,109 kDa
Observed molecular weight	110-130 kDa
GenBank accession number	BC033615
Gene symbol	KIAA0226
Gene ID (NCBI)	9711
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Product Specific Protocols
WB protocol for Rubicon antibody 21444-1-AP	Download protocol
IHC protocol for Rubicon antibody 21444-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Autophagy The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance. Authors - Xing Huang View Article
human	WB,IHC	Cell Mol Immunol Inducible Rubicon facilitates viral replication by antagonizing interferon production. Authors - Yushun Wan KD Validated View Article
rat	WB,IF	Autophagy RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye. Authors - Luis Muniz-Feliciano KD Validated View Article
		Genes Dev USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1. Authors - Daichao Xu View Article
human	WB	Autophagy STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I. Authors - Cefan Zhou View Article