Rubicon Polyclonal antibody

• Featured Product
• KD/KO Validated

Rubicon Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 21444-1-AP

Synonyms

Baron, KIAA0226, RUBCN, Rubicon

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Validation Data Gallery

• 

  WB analysis of mouse liver using 21444-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21444-1-AP (Rubicon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells
• Positive IHC detected in: human testis tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

The immunogen of 21444-1-AP is Rubicon Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Rubicon fusion protein Ag13715
• Full Name: KIAA0226
• Calculated molecular weight: 972 aa, 109 kDa
• Observed molecular weight: 110-130 kDa
• GenBank accession number: BC033615
• Gene symbol: KIAA0226
• Gene ID (NCBI): 9711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Autophagy; The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.; Authors - Xing Huang
mouse	IF	Int J Biol Sci; 17β-Estradiol promotes LC3B-associated phagocytosis in trained immunity of female mice against sepsis.; Authors - Zhiheng Sun ; KD Validated
human	WB,IHC	Cell Mol Immunol; PMID: 28392573; Authors - Yushun Wan ; KD Validated
rat	WB,IF	Autophagy; RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.; Authors - Luis Muniz-Feliciano ; KD Validated
		Genes Dev; USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1.; Authors - Daichao Xu
human	WB	Autophagy; STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.; Authors - Cefan Zhou

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