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  • KD/KO Validated

DYNC1H1 Polyclonal antibody

DYNC1H1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12345-1-AP

Synonyms

DYNC1H 1



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, mouse brain tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue, human normal colon, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12345-1-AP targets DYNC1H1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse, rat, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYNC1H1 fusion protein Ag2999
Full Name dynein, cytoplasmic 1, heavy chain 1
Calculated Molecular Weight 4646 aa, 532 kDa
Observed Molecular Weight 532 kDa
GenBank Accession NumberBC021297
Gene Symbol DYNC1H1
Gene ID (NCBI) 1778
RRIDAB_2261765
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients..

Protocols

Product Specific Protocols
WB protocol for DYNC1H1 antibody 12345-1-APDownload protocol
IHC protocol for DYNC1H1 antibody 12345-1-APDownload protocol
IF protocol for DYNC1H1 antibody 12345-1-APDownload protocol
IP protocol for DYNC1H1 antibody 12345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

Mechanism of spindle pole organization and instability in human oocytes.

Authors - Chun So
mouseIF

Science

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So
humanWB

Nature

Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.

Authors - Henri-François Renard
  • KD Validated
humanWB

Nat Cell Biol

Recycling of autophagosomal components from autolysosomes by the recycler complex.

Authors - Chuchu Zhou
human

Neuron

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Authors - Andrew Kodani
humanWB

J Cell Biol

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

Authors - Hironori Inaba
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (08-24-2021)

Very good antibody, giving a clear and clean signal at the expected molecular weight.

  • Applications: Western Blot