KIAA0368 Polyclonal antibody

KIAA0368 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25684-1-AP

Synonyms

ECM29, extracellular mutant 29, KIAA0368, KIAA1962, RP11 386D8.2



Tested Applications

Positive WB detected inHEK-293 cells, A549 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25684-1-AP is KIAA0368 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA0368 fusion protein Ag22309
Full Name KIAA0368
Calculated molecular weight 1845 aa, 204 kDa
Observed molecular weight 205 kDa
GenBank accession numberBC021127
Gene symbol KIAA0368
Gene ID (NCBI) 23392
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ecm29 binds specifically to 26 S proteasomes. Ecm29 has been reported to stabilize proteasomes, to remodel proteasomes upon stress, to be involved in membrane-associated localization of proteasomes, and, more recently, to be involved in quality control or assembly of the proteasome. Ecm29 acts as a proteasome inhibitor.

Protocols

Product Specific Protocols
WB protocol for KIAA0368 antibody 25684-1-APDownload protocol
IHC protocol for KIAA0368 antibody 25684-1-APDownload protocol
IF protocol for KIAA0368 antibody 25684-1-APDownload protocol
IP protocol for KIAA0368 antibody 25684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols