KIAA0368 Polyclonal antibody

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  WB analysis of HEK-293 using 25684-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25684-1-AP (KIAA0368 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25684-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25684-1-AP (KIAA0368 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 25684-1-AP

  IP result of anti-KIAA0368 (IP:25684-1-AP, 4ug; Detection:25684-1-AP 1:600) with HEK-293 cells lysate 2600ug.

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  IHC staining of human liver using 25684-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25684-1-AP (KIAA0368 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 25684-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25684-1-AP (KIAA0368 antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HEK-293 using 25684-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25684-1-AP (KIAA0368 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

KIAA0368 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 25684-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ECM29, extracellular mutant 29, KIAA0368, KIAA1962, RP11 386D8.2

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

25684-1-AP targets KIAA0368 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22309

  Product name: Recombinant human KIAA0368 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1501-1845 aa of BC021127

  Sequence: EECNLWTEVWQENVPGSFGGIRLYLQELITITQKALQSQSWKMKAQGAIAMASIAKQTSSLVPPYLGMILTALLQGLAGRTWAGKEELLKAIACVVTACSAELEKSVPNQPSTNEILQAVLKECSKENVKYKIVAISCAADILKATKEDRFQEFSNIVIPLIKKNSLESSGVRTTKNEEENEKEKELQLEYLLGAFESLGKAWPRNAETQRCYRQELCKLMCERLKLSTWKVQLGVLQSMNAFFQGLMLLEEEHADPEALAEILLETCKSITYSLENKTYSSVRTEALSVIELLLKKLEESKQWECLTSECRVLLIESLATMEPDSRPELQEKAALLKKTLENLE

• Full Name: KIAA0368
• Calculated Molecular Weight: 1845 aa, 204 kDa
• Observed Molecular Weight: 205 kDa
• GenBank Accession Number: BC021127
• Gene Symbol: KIAA0368
• Gene ID (NCBI): 23392
• RRID: AB_2880193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5VYK3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ecm29 binds specifically to 26 S proteasomes. Ecm29 has been reported to stabilize proteasomes, to remodel proteasomes upon stress, to be involved in membrane-associated localization of proteasomes, and, more recently, to be involved in quality control or assembly of the proteasome. Ecm29 acts as a proteasome inhibitor.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Nanomicro Lett; Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling; Authors - Junji Ren