MRPP3 Polyclonal antibody

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  WB analysis of mouse brain using 20959-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 20959-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20959-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20959-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20959-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20959-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA0391 antibody 20959-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 20959-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA0391 antibody 20959-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

MRPP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 20959-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0391, EC:3.1.26.5, Mitochondrial ribonuclease P catalytic subunit, PRORP, Protein only RNase P catalytic subunit

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, mouse brain tissue, mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20959-1-AP targets MRPP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15196

  Product name: Recombinant human KIAA0391 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 230-583 aa of BC032221

  Sequence: LLLLEDIKKVITPSKKNYNDCIQGALLHQDVNTAWNLYQELLGHDIVPMLETLKAFFDFGKDIKDDNYSNKLLDILSYLRNNQLYPGESFAHSIKTWFESVPGKQWKGQFTTVRKSGQCSGCGKTIESIQLSPEEYECLKGKIMRDVIDGGDQYRKTTPQELKRFENFIKSRPPFDVVIDGLNVAKMFPKVRESQLLLNVVSQLAKRNLRLLVLGRKHMLRRSSQWSRDEMEEVQKQASCFFADDISEDDPFLLYATLHSGNHCRFITRDLMRDHKACLPDAKTQRLFFKWQQGHQLAIVNRFPGSKLTFQRILSYDTVVQTTGDSWHIPYDEDLVERCSCEVPTKWLCLHQKT

• Full Name: KIAA0391
• Calculated Molecular Weight: 583 aa, 67 kDa
• Observed Molecular Weight: 65-67 kDa, 56 kDa
• GenBank Accession Number: BC032221
• Gene Symbol: MRPP3
• Gene ID (NCBI): 9692
• RRID: AB_10860109
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15091
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694). Western detected isoforms of MRPP3 as 67 kDa, 56 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Metab; Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.; Authors - Daniel F Bogenhagen