KIAA0513 Polyclonal antibody

KIAA0513 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21478-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis of mouse brain using 21478-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21478-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21478-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 21478-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21478-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 21478-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21478-1-AP (KIAA0513 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 21478-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21478-1-AP (KIAA0513 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21478-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA0513 antibody 21478-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human testis tissue, mouse cerebellum tissue, mouse kidney tissue, mouse testis tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21478-1-AP targets KIAA0513 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIAA0513 fusion protein Ag15624
• Full Name: KIAA0513
• Calculated Molecular Weight: 411 aa, 47 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC030280
• Gene Symbol: KIAA0513
• Gene ID (NCBI): 9764
• RRID: AB_10755300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA0513 was identified as upregulated in subjects with schizophrenia. The mRNA of KIAA0513 is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. The interacting protein analysis indicated that KIAA0513 is involved in both synaptic and apoptotic signaling. (PMID: 17010949) Several isoforms of KIAA0513 (47/46/34 kDa) exist due to the alternative splicing. This antibody is expected to recognize all of isoforms of KIAA0513.