KIAA0907 Polyclonal antibody

KIAA0907 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25419-1-AP

Synonyms

BLOM7, KHDC4, KIAA0907, RP11 336K24.1, UPF0469 protein KIAA0907

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Validation Data Gallery

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  WB analysis using 25419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25419-1-AP (KIAA0907 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 25419-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25419-1-AP (KIAA0907 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 25419-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25419-1-AP (KIAA0907 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, PC-13 cells, mouse liver tissue, mouse small intestine tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25419-1-AP targets KIAA0907 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIAA0907 fusion protein Ag22217
• Full Name: KIAA0907
• Calculated Molecular Weight: 614 aa, 65 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC062637
• Gene Symbol: KIAA0907
• Gene ID (NCBI): 22889
• RRID: AB_2880070
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA0907, also named as BLOM7, is 614 amino acid protein, which belongs to the UPF0469 family. KIAA0907 exists as four alternative splicing isoforms and its residues is modified by phosphorylase. KIAA0907 are frequently observed in lung cancer cells.