TMEM131L Polyclonal antibody

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• TMEM131L Polyclonal antibody (23360-1-AP)

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  WB analysis using 23360-1-AP

  Ramos cells lysates were subjected to SDS PAGE followed by western blot with 23360-1-AP (TMEM131L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 23360-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23360-1-AP (TMEM131L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 23360-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23360-1-AP (KIAA0922 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 23360-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23360-1-AP (KIAA0922 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 23360-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KIAA0922 antibody (23360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U-251 using 23360-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using KIAA0922 antibody (23360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

TMEM131L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23360-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0922, Transmembrane protein 131 like, Transmembrane protein 131-like

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Tested Applications

• Positive WB detected in: Ramos cells, HL-60 cells, unboiled HL-60 cells
• Positive IHC detected in: mouse colon tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23360-1-AP targets TMEM131L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19898

  Product name: Recombinant human KIAA0922 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1110-1461 aa of BC131505

  Sequence: WCIQESTREVCKADAEIASSLPAAQREAEGYYQKPEKKCVDKFCSDSSSDCGSSSGSVRASRGSWGSWSSTSSSDGDKKPMVDAQHFLPAGDSVSQNDFPSEAPISLNLSHNICNPMTVNSLPQYAEPSCPSLPAGPTGVEEDKGLYSPGDLWPTPPVCVTSSLNCTLENGVPCVIQESAPVHNSFIDWSATCEGQFSSAYCPLELNDYNAFPEENMNYANGFPCPADVQTDFIDHNSQSTWNTPPNMPAAWGHASFISSPPYLTSTRSLSPMSGLFGSIWAPQSDVYENCCPINPTTEHSTHMENQAVVCKEYYPGFNPFRAYMNLDIWTTTANRNANFPLSRDSSYCGNV

• Full Name: KIAA0922
• Calculated Molecular Weight: 1609 aa, 179 kDa
• Observed Molecular Weight: 179 kDa
• GenBank Accession Number: BC131505
• Gene Symbol: KIAA0922
• Gene ID (NCBI): 23240
• RRID: AB_3085709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A2VDJ0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA0922 is also named as TMEM131L (Transmembrane protein 131-like). Human KIAA0922/TMEM131L encodes a transmembrane protein, TMEM131L, that regulates the canonical Wnt/β-catenin signaling pathway by eliciting the lysosome-dependent degradation of phosphorylated LRP6 co-receptor (PMID: 28347816).