KIAA1279 Polyclonal antibody

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  WB analysis using 25653-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25653-1-AP (KIAA1279 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25653-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25653-1-AP (KIAA1279 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25653-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25653-1-AP (KIAA1279 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 25653-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 25653-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 25653-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 25653-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

KIAA1279 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25653-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

KBP, KIAA1279, KIF1 binding protein, TTC20

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, NIH/3T3 cells
• Positive IHC detected in: mouse kidney tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

25653-1-AP targets KIAA1279 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22234

  Product name: Recombinant human KIAA1279 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 272-621 aa of BC012180

  Sequence: CLSAANVIFGQTGKISATEDTPEAEGEVPELYHQRKGEIARCWIKYCLTLMQNAQLSMQDNIGELDLDKQSELRALRKKELDEEESIRKKAVQFGTGELCDAISAVEEKVSYLRPLDFEEARELFLLGQHYVFEAKEFFQIDGYVTDHIEVVQDHSALFKVLAFFETDMERRCKMHKRRIAMLEPLTVDLNPQYYLLVNRQIQFEIAHAYYDMMDLKVAIADRLRDPDSHIVKKINNLNKSALKYYQLFLDSLRDPNKVFPEHIGEDVLRPAMLAKFRVARLYGKIITADPKKELENLATSLEHYKFIVDYCEKHPEAAQEIEVELELSKEMVSLLPTKMERFRTKMALT

• Full Name: KIAA1279
• Calculated Molecular Weight: 621 aa, 72 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC012180
• Gene Symbol: KIAA1279
• Gene ID (NCBI): 26128
• RRID: AB_2880178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96EK5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Front Mol Neurosci; Multiple Functions of KBP in Neural Development Underlie Brain Anomalies in Goldberg-Shprintzen Syndrome.; Authors - Hsin-Yun Chang ; KD Validated
mouse	WB, IF	Reproduction; KIF1-binding protein interacts with KIF3A in haploid male germ cells.; Authors - Mari S Lehti
		Life Sci Alliance; Axonal transport of CHMP2b is regulated by kinesin-binding protein and disrupted by CHMP2bintron5; Authors - Konner R Kirwan