KIAA1468 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• KIAA1468 Polyclonal antibody (18993-1-AP)

KIAA1468 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 18993-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

RELCH, HsT3308, HsT885, LisH domain and HEAT repeat-containing protein KIAA1468, RAB11 binding and LisH domain, coiled-coil and HEAT repeat-containing

• 

  WB analysis using 18993-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18993-1-AP (KIAA1468 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 18993-1-AP

  IP result of anti-KIAA1468 (IP:18993-1-AP, 4ug; Detection:18993-1-AP 1:500) with mouse brain tissue lysate 1280 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 18993-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 18993-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 18993-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 18993-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat ovary using 18993-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18993-1-AP targets KIAA1468 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: KIAA1468
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 100-135 kDa
• GenBank Accession Number: NM_020854
• Gene Symbol: KIAA1468
• Gene ID (NCBI): 57614
• RRID: AB_10598164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P260
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RELCH/KIAA1468, which is a newly identified Rab11 effector protein, tethers the recycling endosomes (REs) and trans-Golgi network (TGN) membranes by binding TGN-localized oxysterol-binding protein (OSBP) and promotes OSBP-dependent nonvesicular cholesterol transport from REs to the TGN.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse, pig	IP	Cell Rep; A cross-vertebrate brain protein interaction map identifies conserved neural and non-neural complexes.; Authors - Vy Dang