NCKAP5L Polyclonal antibody

NCKAP5L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21392-1-AP

Synonyms

Cep169, FP1193, KIAA1602, Nck associated protein 5 like, NCKAP5L

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Validation Data Gallery

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  WB analysis of HeLa using 21392-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21392-1-AP (NCKAP5L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 21392-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 21392-1-AP (NCKAP5L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 21392-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 21392-1-AP (NCKAP5L antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21392-1-AP targets NCKAP5L in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCKAP5L fusion protein Ag16050
• Full Name: KIAA1602
• Calculated Molecular Weight: 1334 aa, 139 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC110599
• Gene Symbol: NCKAP5L
• Gene ID (NCBI): 57701
• RRID: AB_10860259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NCKAP5L, also named as KIAA1602, has 4 isoforms. The target band is about 139-145 kDa for phosphorylation.