CEP44 Polyclonal antibody

• Featured Product
• KD/KO Validated

CEP44 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 24457-1-AP

Synonyms

PS1TP3, HBV PreS1-transactivated protein 3, Centrosomal protein of 44 kDa

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Validation Data Gallery

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  WB analysis using 24457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24457-1-AP (CEP44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24457-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24457-1-AP (CEP44 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 24457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24457-1-AP (CEP44 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24457-1-AP (CEP44 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 24457-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24457-1-AP (CEP44 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 24457-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24457-1-AP (CEP44 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MDCK using 24457-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CEP44 antibody (24457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, Jurkat cells, MCF-7 cells
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

24457-1-AP targets CEP44 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP44 fusion protein Ag19961
• Full Name: KIAA1712
• Calculated Molecular Weight: 390 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC038667
• Gene Symbol: CEP44
• Gene ID (NCBI): 80817
• RRID: AB_2879557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP44, also named as KIAA1712 and PS1TP3, localizes to centrosomes, spindle poles, and weakly in midbody. It is a 44 kDa centrosome protein.

Publications

Species	Application	Title
human	IF	J Cell Biol; Centrosomal organization of Cep152 provides flexibility in Plk4 and procentriole positioning; Authors - Catherine Sullenberger
human	WB,IF,IP	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin; Authors - Delowar Hossain ; KD Validated
human	WB,IF	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin.; Authors - Delowar Hossain ; KD Validated
		Mol Biol Cell; "Roles for ELMOD2 and Rootletin in Ciliogenesis".; Authors - Rachel E Turn
mouse	WB	Cell Rep; A program of successive gene expression in mouse one-cell embryos; Authors - Maki Asami
human	IF	Nat Commun; Molecular basis promoting centriole triplet microtubule assembly; Authors - Yutaka Takeda