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CEP44 Polyclonal antibody

CEP44 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 24457-1-AP

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Centrosomal protein of 44 kDa, HBV PreS1-transactivated protein 3, PS1TP3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHCIF/ICC

WB analysis using 24457-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24457-1-AP (CEP44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 24457-1-AP
IP result of anti-CEP44 (IP:24457-1-AP, 4ug; Detection:24457-1-AP 1:3000) with HeLa cells lysate 1640 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 24457-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24457-1-AP (CEP44 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 24457-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24457-1-AP (CEP44 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 24457-1-AP
Immunohistochemical analysis of paraffin-embedded human brain slide using 24457-1-AP (CEP44 antibody at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 24457-1-AP
Immunohistochemical analysis of paraffin-embedded human brain slide using 24457-1-AP (CEP44 antibody at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MDCK using 24457-1-AP
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CEP44 antibody (24457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	MDCK cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24457-1-AP targets CEP44 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity	human, canine
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19961 Product name: Recombinant human KIAA1712 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC038667 Sequence: MATGDLKRSLRNLEQVLRLLNYPEEVDCVGLIKGDPAASLPIISYSFTSYSPYVTELIMESNVELIAKNDLRFIDAVYKLLRDQFNYKPILTKKQFIQCGFAEWKIQIVCDILNCVMKKHKELSSLQKIPSQQRKKISSGKSEPPLGNEKISAEAVGVDISGRFMTSGKKKAVVIRHLYNEDNVDISEDTLSPITDVNEAVDVSDLNATEIKMPEVKVPEIKAEQQDVNVNPEITALQTMLAECQENLKKLTSIEKRLDCLEQKMKGKVMVDENTWTNLLSRVTLLETEMLLSKKNDEFIEFNEVSEDYASCSDMDLLNPHRKSEVERPASIPLSSGYSTASSDSTPRAS Predict reactive species
Full Name	KIAA1712
Calculated Molecular Weight	390 aa, 44 kDa
Observed Molecular Weight	44 kDa
GenBank Accession Number	BC038667
Gene Symbol	CEP44
Gene ID (NCBI)	80817
RRID	AB_2879557
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9C0F1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP44, also named as KIAA1712 and PS1TP3, localizes to centrosomes, spindle poles, and weakly in midbody. It is a 44 kDa centrosome protein.

Protocols

Product Specific Protocols
IF protocol for CEP44 antibody 24457-1-AP	Download protocol
IHC protocol for CEP44 antibody 24457-1-AP	Download protocol
IP protocol for CEP44 antibody 24457-1-AP	Download protocol
WB protocol for CEP44 antibody 24457-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The CEP44 Polyclonal antibody (Cat# 24457-1-AP) has 12 citations published in journals including Cell, Nature Communications, Cell Reports, Journal of Cell Biology, Journal of Cell Science, Molecular Biology of the Cell, Cytoskeleton, MicroPubl Biology, and eLife. Research fields span centriole assembly and duplication, centrosome cohesion, ciliogenesis, early embryo gene expression, O-GlcNAcylation regulation, and sperm centriole architecture, primarily in human and mouse systems.

Species	Application	Title
human	IF	Cell Time-series reconstruction of the molecular architecture of human centriole assembly Authors - Marine H Laporte View Article
human	IF	Nat Commun Molecular basis promoting centriole triplet microtubule assembly Authors - Yutaka Takeda View Article
human	IF	Nat Commun The A-C linker controls centriole structural integrity and duplication Authors - Lorène Bournonville View Article
		Cell Rep 3D nanoscale reconstruction of human airway multiciliogenesis reveals cellular architecture remodeling and cilia-mitochondria link via rootlets. Authors - Aaran Vijayakumaran View Article
mouse	WB	Cell Rep A program of successive gene expression in mouse one-cell embryos Authors - Maki Asami View Article
human	IF	J Cell Biol Centrosomal organization of Cep152 provides flexibility in Plk4 and procentriole positioning Authors - Catherine Sullenberger View Article