KIAA1715 Polyclonal antibody

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  WB analysis using 30857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30857-1-AP (KIAA1715 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 30857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30857-1-AP (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KIAA1715 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30857-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

DKFZp686G1442, KIAA1715, LNP, Protein lunapark, Ul, ulnaless

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, K-562 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30857-1-AP targets KIAA1715 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33996

  Product name: Recombinant human KIAA1715 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 101-428 aa of BC105132

  Sequence: RTERNNEALDDLKSQRKKILEEVMEKETYKTAKLILERFDPDSKKAKECEPPSAGAAVTARPGQEIRQRTAAQRNLSPTPASPNQGPPPQVPVSPGPPKDSSAPGGPPERTVTPALSSNVLPRHLGSPATSVPGMGLHPPGPPLARPILPRERGALDRIVEYLVGDGPQNRYALICQQCFSHNGMALKEEFEYIAFRCAYCFFLNPARKTRPQAPRLPEFSFEKRQVVEGSSSVGPLPSGSVLSSDNQFNEESLEHDVLDDNTEQTDDKIPATEQTNQVIEKASDSEEPEEKQETENEEASVIETNSTVPGADSIPDPELSGESLTAE

• Full Name: KIAA1715
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC105132
• Gene Symbol: KIAA1715
• Gene ID (NCBI): 80856
• RRID: AB_3086424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9C0E8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).