Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, human brain tissue, Raji cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human ovarian cancer, human ovary cancer tissue, human placenta tissue, mouse cerebellum tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21856-1-AP targets KIDINS220 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16448 Product name: Recombinant human KIDINS220 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1374-1672 aa of BC130610 Sequence: LDPITEEDEKSDQSGSKLLPGKKSSERSSLFQTDLKLKGSGLRYQKLPSDEDESGTEESDNTPLLKDDKDRKAEGKVERVPKSPEHSAEPIRTFIKAKEYLSDALLDKKDSSDSGVRSSESSPNHSLHNEVADDSQLEKANLIELEDDSHSGKRGIPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEENWPACQKAYNLNRTPSTVTLNNNSAPANRANQNFDEMEGIRETSQVILRPSSSPNPTTIQNENLKSMTHKRSQRSSYTRLSKDPPELHAAASSESTGFGEERESIL Predict reactive species |
| Full Name | kinase D-interacting substrate, 220kDa |
| Calculated Molecular Weight | 1672 aa, 187 kDa |
| Observed Molecular Weight | 186-197 kDa |
| GenBank Accession Number | BC130610 |
| Gene Symbol | KIDINS220 |
| Gene ID (NCBI) | 57498 |
| RRID | AB_10836934 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Kidins220 (Kinase D-interacting substrate 220 kD) is a crucial mediator of signal transduction in neural tissues. Also it named ARMS(Ankyrin Repeat-Rich Membrane Spanning) due to the presence of ankyrin repeats and transmembrane domains together with other binding motifs. It is a multidomain transmembrane protein that not only transduces signaling mediated by the receptor tyrosine kinase (RTK), but also regulates the stability of cytoskeletal network through a number of cytoskeleton-associated proteins, including Septin5, MAPs , and stathmin family members. KIDINS220 exists some isoforms with MV 194 kDa, 185 kDa, 115 kDa, and 60 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIDINS220 antibody 21856-1-AP | Download protocol |
| IP protocol for KIDINS220 antibody 21856-1-AP | Download protocol |
| WB protocol for KIDINS220 antibody 21856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody SNX27 (Cat# 21856-1-AP) has 10 citations published in journals including Nature Cell Biology, Nature Communications (×3), Science Advances, Journal of Experimental Medicine, PNAS, Bone Research, and Frontiers in Immunology (×2). Research fields span endosomal/retromer trafficking, mechanotransduction and glycolysis, degron tag technology, RAB GTPase regulation, thymic iNKT cell development, B cell signaling, endocytic recycling, and IgLON5 autoimmunity.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. | ||
Bone Res Matrix stiffness regulates nucleus pulposus cell glycolysis by MRTF-A-dependent mechanotransduction | ||
Nat Commun Mapping of endosomal proximity proteomes reveals Retromer as a hub for RAB GTPase regulation | ||
Nat Commun Systematic profiling of conditional degron tag technologies for target validation studies | ||
Sci Adv Kidins220 and Aiolos promote thymic iNKT cell development by reducing TCR signals |
Reviews
What customers say
One verified customer review (5/5 stars) reports successful use of this antibody for Western Blot on H4 cell lysate at a 1:1000 dilution. The reviewer used 3% BSA in TBS-Tween (0.1%) as the diluent and confirmed clear detection of aKIDINS220 by SDS-PAGE. Overall, the single review reflects positive performance with no reported issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kincaid (Verified Customer) (06-02-2024) | H4 cell lysate run on SDS-PAGE gel. aKIDINS220 diluted in 3% BSA in TBS-tween (0.1%).
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