KIDINS220 Monoclonal antibody

• 

  WB analysis using 66748-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66748-1-Ig (KIDINS220 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

KIDINS220 Monoclonal Antibody for WB, ELISA

Cat No. 66748-1-Ig

Clone No.3G2D8

Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

ARMS, KIAA1250, KIDINS220

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66748-1-Ig targets KIDINS220 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17624

  Product name: Recombinant human KIDINS220 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1374-1672 aa of BC130610

  Sequence: LDPITEEDEKSDQSGSKLLPGKKSSERSSLFQTDLKLKGSGLRYQKLPSDEDESGTEESDNTPLLKDDKDRKAEGKVERVPKSPEHSAEPIRTFIKAKEYLSDALLDKKDSSDSGVRSSESSPNHSLHNEVADDSQLEKANLIELEDDSHSGKRGIPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEENWPACQKAYNLNRTPSTVTLNNNSAPANRANQNFDEMEGIRETSQVILRPSSSPNPTTIQNENLKSMTHKRSQRSSYTRLSKDPPELHAAASSESTGFGEERESIL

• Full Name: kinase D-interacting substrate, 220kDa
• Calculated Molecular Weight: 1672 aa, 187 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC130610
• Gene Symbol: KIDINS220
• Gene ID (NCBI): 57498
• RRID: AB_2882095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9ULH0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kidins220 (Kinase D-interacting substrate 220 kD) is a crucial mediator of signal transduction in neural tissues. Also it named ARMS(Ankyrin Repeat-Rich Membrane Spanning) due to the presence of ankyrin repeats and transmembrane domains together with other binding motifs. It is a multidomain transmembrane protein that not only transduces signaling mediated by the receptor tyrosine kinase (RTK), but also regulates the stability of cytoskeletal network through a number of cytoskeleton-associated proteins, including Septin5, MAPs , and stathmin family members. KIDINS220 exists some isoforms with MV 194 kDa, 185 kDa, 115 kDa, and 60 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Synergistic activation of the human phosphate exporter XPR1 by KIDINS220 and inositol pyrophosphate; Authors - Peng Zuo