Eg5 Polyclonal antibody

Eg5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23333-1-AP

Synonyms

EG5, HKSP, KIF11, kinesin family member 11, Kinesin like protein 1, Kinesin like protein KIF11, KNSL1, TR interacting protein 5, TRIP 5, TRIP5



Tested Applications

Positive WB detected inPC-13 cells, MCF-7 cells, Raji cells, HEK-293 cells, Jurkat cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23333-1-AP is Eg5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Eg5 fusion protein Ag18695
Full Name kinesin family member 11
Calculated molecular weight 1056 aa, 119 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC136474
Gene symbol KIF11
Gene ID (NCBI) 3832
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.

Protocols

Product Specific Protocols
WB protocol for Eg5 antibody 23333-1-APDownload protocol
IHC protocol for Eg5 antibody 23333-1-APDownload protocol
IF protocol for Eg5 antibody 23333-1-APDownload protocol
IP protocol for Eg5 antibody 23333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Involvement of C-terminal truncation mutation of kinesin-5 in resistance to kinesin-5 inhibitor.

Authors - Eri Saeki
humanWB

Am J Transl Res

Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma.

Authors - Xishan Li
humanWB

Mol Carcinog

Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.

Authors - Benfang Wang
  • KD Validated
humanIHC

Int J Cancer

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Authors - Fangqiu Fu