|Positive WB detected in||HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human lung cancer tissue, human endometrial cancer tissue, human prostate hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 23333-1-AP is Eg5 Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Eg5 fusion protein Ag18695|
|Full Name||kinesin family member 11|
|Calculated molecular weight||1056 aa, 119 kDa|
|Observed molecular weight||120-130 kDa|
|GenBank accession number||BC136474|
|Gene ID (NCBI)||3832|
|Purification Method||Antigen Affinity purified|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.
|Product Specific Protocols|
|WB protocol for Eg5 antibody 23333-1-AP||Download protocol|
|IHC protocol for Eg5 antibody 23333-1-AP||Download protocol|
|IF protocol for Eg5 antibody 23333-1-AP||Download protocol|
|IP protocol for Eg5 antibody 23333-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Involvement of C-terminal truncation mutation of kinesin-5 in resistance to kinesin-5 inhibitor.
KIF11 and KIF15 mitotic kinesins are potential therapeutic vulnerabilities for malignant peripheral nerve sheath tumors.
Am J Transl Res
Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma.
Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.
Int J Cancer
Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iram (Verified Customer) (01-15-2021)
Eg5 antibody giving a very clean bands and good staining