Eg5 Polyclonal antibody

• Featured Product
• KD/KO Validated

Eg5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (1)

Cat no : 23333-1-AP

Synonyms

KIF11, KNSL1, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Kinesin-like protein KIF11

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Validation Data Gallery

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  WB analysis using 23333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23333-1-AP (Eg5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 23333-1-AP

  IP result of anti-Eg5 (IP:23333-1-AP, 4ug; Detection:23333-1-AP 1:1000) with Raji cells lysate 2000ug.

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  IHC staining of human lung cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 23333-1-AP (Eg5 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 23333-1-AP (Eg5 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 23333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (23333-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 23333-1-AP

  Immunofluorescent analysis of HepG2 cells using 23333-1-AP (Eg5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human lung cancer tissue, human endometrial cancer tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IHC: See 9 publications below
• IF: See 6 publications below

Product Information

23333-1-AP targets Eg5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Eg5 fusion protein Ag18695
• Full Name: kinesin family member 11
• Calculated Molecular Weight: 1056 aa, 119 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC136474
• Gene Symbol: Eg5
• Gene ID (NCBI): 3832
• RRID: AB_2716831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.

Publications

Species	Application	Title
mouse	IF	Br J Cancer; Albendazole inhibits colon cancer progression and therapy resistance by targeting ubiquitin ligase RNF20; Authors - Iram Fatima
mouse	IHC	Int J Oncol; Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway; Authors - Ting Xue
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	Int J Biol Sci; KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer.; Authors - Dang Wei
human	WB	Mol Carcinog; Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.; Authors - Benfang Wang ; KD Validated
human	WB	J Cancer Res Clin Oncol; KIF11 serves as a cell cycle mediator in childhood acute lymphoblastic leukemia; Authors - Liwen Zhu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

Eg5 antibody giving a very clean bands and good staining

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC and IF
• Cell Tissue Type: Human colon cancer cells and tissue