Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human lung cancer tissue, human endometrial cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23333-1-AP targets Eg5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18695 Product name: Recombinant human KIF11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 707-1052 aa of BC136474 Sequence: LTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQKFCADSDGFSQELRNFNQEGTKLVEESVKHSDKLNGNLEKISQETEQRCESLNTRTVYFSEQWVSSLNEREQELHNLLEVVSQCCEASSSDITEKSDGRKAAHEKQHNIFLDQMTIDEDKLIAQNLELNETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRA Predict reactive species |
| Full Name | kinesin family member 11 |
| Calculated Molecular Weight | 1056 aa, 119 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | BC136474 |
| Gene Symbol | Eg5 |
| Gene ID (NCBI) | 3832 |
| RRID | AB_2716831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P52732 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Eg5 antibody 23333-1-AP | Download protocol |
| IHC protocol for Eg5 antibody 23333-1-AP | Download protocol |
| IP protocol for Eg5 antibody 23333-1-AP | Download protocol |
| WB protocol for Eg5 antibody 23333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Eg5 Polyclonal antibody (Cat# 23333-1-AP) has 27 citations published in journals including Cell Death & Disease, International Journal of Biological Sciences, Journal of Biological Chemistry, Clinical and Translational Medicine, British Journal of Cancer, and PLoS ONE. Research fields span oncology (lung, breast, ovarian, pancreatic, glioma, and other cancers), cell cycle regulation, mitotic spindle biology, drug resistance mechanisms, and signaling pathways such as Wnt/β-catenin and PI3K/AKT.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Knockdown of TACC3 inhibits tumor cell proliferation and increases chemosensitivity in pancreatic cancer | ||
Cell Death Dis The therapeutic effect of KSP inhibitors in preclinical models of cholangiocarcinoma
| ||
Int J Biol Sci KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. | ||
Phytomedicine Targeting KIF11 degradation: Oxypeucedanin inhibits lung adenocarcinoma progression by disrupting the MCM2-CDK1 signaling pathway. | ||
J Transl Med Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer
|
Reviews
What customers say
The single review for this Eg5 antibody (lot 00061731) rates it 5 stars. The reviewer used it for Western Blot, Immunohistochemistry, and Immunofluorescence on human colon cancer cells and tissue, with dilutions of 1:1000 for Western and 1:100 for IHC and IF. They reported very clean bands and good staining, indicating strong performance across all three applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Iram (Verified Customer) (01-15-2021) | Eg5 antibody giving a very clean bands and good staining
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