|Positive WB detected in||PC-13 cells, MCF-7 cells, Raji cells, HEK-293 cells, Jurkat cells|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human lung cancer tissue, human endometrial cancer tissue, human prostate hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 23333-1-AP is Eg5 Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Eg5 fusion protein Ag18695|
|Full Name||kinesin family member 11|
|Calculated molecular weight||1056 aa, 119 kDa|
|Observed molecular weight||120-130 kDa|
|GenBank accession number||BC136474|
|Gene ID (NCBI)||3832|
|Purification Method||Antigen Affinity purified|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.
|Product Specific Protocols|
|WB protocol for Eg5 antibody 23333-1-AP||Download protocol|
|IHC protocol for Eg5 antibody 23333-1-AP||Download protocol|
|IF protocol for Eg5 antibody 23333-1-AP||Download protocol|
|IP protocol for Eg5 antibody 23333-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Involvement of C-terminal truncation mutation of kinesin-5 in resistance to kinesin-5 inhibitor.
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