KIF14 Polyclonal antibody

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• KIF14 Polyclonal antibody (26000-1-AP)

KIF14 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 26000-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Synonyms

KIAA0042, KIF14, kinesin family member 14, Kinesin like protein KIF14

• 

  WB analysis of T-47D using 26000-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293T using 26000-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 26000-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293T using 26000-1-AP

  IP result of anti-KIF14 (IP:26000-1-AP, 4ug; Detection:26000-1-AP 1:300) with HEK-293T cells lysate 4000 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 26000-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26000-1-AP (KIF14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 26000-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26000-1-AP (KIF14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: T-47D cells, HEK-293T cells, HeLa cells
• Positive IP detected in: HEK-293T cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26000-1-AP targets KIF14 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23219

  Product name: Recombinant human KIF14 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1299-1648 aa of BC113742

  Sequence: SDRAIQSLTIQTACAFEQLVVLMKHWLSDLLPCTNIARLEDELRQEVKKLGGYLQLFLQGCCLDISSMIKEAQKNAIQIVQQAVKYVGQLAVLKGSKLHFLENGNNKAASVQEEFMDAVCDGVGLGMKILLDSGLEKAKELQHELFRQCTKNEVTKEMKTNAMGLIRSLENIFAESKIKSFRRQVQEENFEYQDFKRMVNRAPEFLKLKHCLEKAIEIIISALKGCHSDINLLQTCVESIRNLASDFYSDFSVPSTSVGSYESRVTHIVHQELESLAKSLLFCFESEESPDLLKPWETYNQNTKEEHQQSKSSGIDGSKNKGVPKRVYELHGSSAAVSSEECTPSRIQWV

• Full Name: kinesin family member 14
• GenBank Accession Number: BC113742
• Gene Symbol: KIF14
• Gene ID (NCBI): 9928
• RRID: AB_2880327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15058
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-KIF14 antibody (Cat# 26000-1-AP) has 6 total citations, published in Cell Death Discovery, Pharmaceuticals, Journal of Cellular and Molecular Medicine, Carcinogenesis, Journal of Thoracic Disease, and BMC Urology. The research fields are all within oncology, spanning gastric cancer, glioma, anaplastic thyroid carcinoma, gallbladder cancer, lung adenocarcinoma, and clear cell renal cell carcinoma. Applications used include IHC, WB, and IF in human samples.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Cell Death Discov; Identification of tRF-29-79MP9P9NH525 as a biomarker and tumor suppressor of gastric cancer via regulating KIF14/AKT pathway; Authors - Jiaxin Ge
human	IHC	Pharmaceuticals (Basel); Identification of Key Immune and Cell Cycle Modules and Prognostic Genes for Glioma Patients through Transcriptome Analysis; Authors - Kaimin Guo
human	WB,IF	J Cell Mol Med; BUB1/KIF14 complex promotes anaplastic thyroid carcinoma progression by inducing chromosome instability; Authors - Tiefeng Jin
human	IHC	Carcinogenesis; NUF2 and NEK2 promote malignant progression of gallbladder cancer by remodeling the extracellular matrix; Authors - Ming Gao
human	IHC	J Thorac Dis; Exploring diverse programmed cell-death patterns to develop a novel gene signature for predicting the prognosis of lung adenocarcinoma patients; Authors - Zhanming Ma
human	WB	BMC Urol; KIF14 plays a role in the regulation of the cell cycle and has implications for prognosis in clear cell renal cell carcinoma; Authors - Jie Wang