KIF14 Polyclonal antibody

KIF14 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26000-1-AP

Synonyms

KIAA0042, KIF14, kinesin family member 14, Kinesin like protein KIF14

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Validation Data Gallery

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  WB analysis of T-47D using 26000-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 26000-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26000-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293T using 26000-1-AP

  IP result of anti-KIF14 (IP:26000-1-AP, 4ug; Detection:26000-1-AP 1:300) with HEK-293T cells lysate 4000 ug.

• 

  IHC staining of human prostate cancer using 26000-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26000-1-AP (KIF14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 26000-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26000-1-AP (KIF14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: T-47D cells, HEK-293T cells, HeLa cells
• Positive IP detected in: HEK-293T cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 26000-1-AP is KIF14 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF14 fusion protein Ag23219
• Full Name: kinesin family member 14
• GenBank Accession Number: BC113742
• Gene Symbol: KIF14
• Gene ID (NCBI): 9928
• RRID: AB_2880327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Thorac Dis; Exploring diverse programmed cell-death patterns to develop a novel gene signature for predicting the prognosis of lung adenocarcinoma patients; Authors - Zhanming Ma
human	WB,IF	J Cell Mol Med; BUB1/KIF14 complex promotes anaplastic thyroid carcinoma progression by inducing chromosome instability; Authors - Tiefeng Jin