KIF14 Polyclonal antibody

• 

  WB analysis of T-47D using 26000-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26000-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 26000-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293T using 26000-1-AP

  IP result of anti-KIF14 (IP:26000-1-AP, 4ug; Detection:26000-1-AP 1:300) with HEK-293T cells lysate 4000 ug.

• 

  IHC staining of human prostate cancer using 26000-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26000-1-AP (KIF14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 26000-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26000-1-AP (KIF14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KIF14 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 26000-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Synonyms

KIAA0042, KIF14, kinesin family member 14, Kinesin like protein KIF14

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Tested Applications

• Positive WB detected in: T-47D cells, HEK-293T cells, HeLa cells
• Positive IP detected in: HEK-293T cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

26000-1-AP targets KIF14 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23219

  Product name: Recombinant human KIF14 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1299-1648 aa of BC113742

  Sequence: SDRAIQSLTIQTACAFEQLVVLMKHWLSDLLPCTNIARLEDELRQEVKKLGGYLQLFLQGCCLDISSMIKEAQKNAIQIVQQAVKYVGQLAVLKGSKLHFLENGNNKAASVQEEFMDAVCDGVGLGMKILLDSGLEKAKELQHELFRQCTKNEVTKEMKTNAMGLIRSLENIFAESKIKSFRRQVQEENFEYQDFKRMVNRAPEFLKLKHCLEKAIEIIISALKGCHSDINLLQTCVESIRNLASDFYSDFSVPSTSVGSYESRVTHIVHQELESLAKSLLFCFESEESPDLLKPWETYNQNTKEEHQQSKSSGIDGSKNKGVPKRVYELHGSSAAVSSEECTPSRIQWV

• Full Name: kinesin family member 14
• GenBank Accession Number: BC113742
• Gene Symbol: KIF14
• Gene ID (NCBI): 9928
• RRID: AB_2880327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15058
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Pharmaceuticals (Basel); Identification of Key Immune and Cell Cycle Modules and Prognostic Genes for Glioma Patients through Transcriptome Analysis; Authors - Kaimin Guo
human	WB,IF	J Cell Mol Med; BUB1/KIF14 complex promotes anaplastic thyroid carcinoma progression by inducing chromosome instability; Authors - Tiefeng Jin
human	IHC	J Thorac Dis; Exploring diverse programmed cell-death patterns to develop a novel gene signature for predicting the prognosis of lung adenocarcinoma patients; Authors - Zhanming Ma
human	IHC	Cell Death Discov; Identification of tRF-29-79MP9P9NH525 as a biomarker and tumor suppressor of gastric cancer via regulating KIF14/AKT pathway; Authors - Jiaxin Ge
human	IHC	Carcinogenesis; NUF2 and NEK2 promote malignant progression of gallbladder cancer by remodeling the extracellular matrix; Authors - Ming Gao
human	WB	BMC Urol; KIF14 plays a role in the regulation of the cell cycle and has implications for prognosis in clear cell renal cell carcinoma; Authors - Jie Wang