KIF2C Polyclonal antibody

KIF2C Polyclonal Antibody for IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12139-1-AP

Synonyms

KIF2C, kinesin family member 2C, Kinesin like protein 6, Kinesin like protein KIF2C, KNSL6, MCAK

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Validation Data Gallery

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  IP experiment of mouse testis using 12139-1-AP

  IP result of anti-KIF2C (IP:12139-1-AP, 3ug; Detection:12139-1-AP 1:500) with mouse testis tissue lysate 8000ug.

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  IF Staining of HeLa using 12139-1-AP

  Immunofluorescent analysis of HeLa cells using 12139-1-AP (KIF2C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IP detected in: mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

12139-1-AP targets KIF2C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF2C fusion protein Ag2786
• Full Name: kinesin family member 2C
• Calculated Molecular Weight: 725 aa, 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC014924
• Gene Symbol: KIF2C
• Gene ID (NCBI): 11004
• RRID: AB_2877829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Elife; KIF2C regulates synaptic plasticity and cognition in mice through dynamic microtubule depolymerization.; Authors - Rui Zheng
mouse	IF	J Physiol; Ablation of KIF2C in Purkinje cells impairs metabotropic glutamate receptor trafficking and motor coordination in male mice; Authors - Rui Zheng
human	IF	Front Cell Dev Biol; Integrative Analysis of Biomarkers Through Machine Learning Identifies Stemness Features in Colorectal Cancer.; Authors - Ran Wei
human	IHC	Front Oncol; Novel prognostic biomarkers in nasopharyngeal carcinoma unveiled by mega-data bioinformatics analysis; Authors - Yishuai Tan
human	WB	Eur J Med Res; Potential 'anti-cancer' effects of esketamine on proliferation, apoptosis, migration and invasion in esophageal squamous carcinoma cells; Authors - Chao Li
human		Carcinogenesis; Systematically characterize the clinical and biological significances of 1p19q genes in 1p/19q non-codeletion glioma.; Authors - Rui-Chao Chai