KIF2C Polyclonal antibody

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• KIF2C Polyclonal antibody (28372-1-AP)

KIF2C Polyclonal Antibody for WB, IHC, ELISA

Cat No. 28372-1-AP Copied!

Publications (16)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Kinesin-like protein 6, Kinesin-like protein KIF2C, KNSL6, MCAK, Mitotic centromere-associated kinesin

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  WB analysis using 28372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28372-1-AP (KIF2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 28372-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28372-1-AP (KIF2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 28372-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28372-1-AP (KIF2C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 28372-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 28372-1-AP (KIF2C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 28372-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 28372-1-AP (KIF2C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 28372-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28372-1-AP (KIF2C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat small intestine using 28372-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28372-1-AP (KIF2C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 28372-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28372-1-AP (KIF2C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: DU 145 cells, PC-3 cells, mouse testis, rat testis
• Positive IHC detected in: mouse testis tissue, rat testis tissue, mouse testis tissue, rat small intestine tissue, human placenta tissue, human appendicitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28372-1-AP targets KIF2C in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29009

  Product name: Recombinant human KIF2C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-186 aa of BC014924

  Sequence: MAMDSSLQARLFPGLAIKIQRSNGLIHSANVRTVNLEKSCVSVEWAEGGATKGKEIDFDDVAAINPELLQLLPLHPKDNLPLQENVTIQKQKRRSVNSKIPAPKESLRSRSTRMSTVSELRITAQENDMEVELPAAANSRKQFSVPPAPTRPSCPAVAEIPLRMVSEEMEEQVHSIRGSSSANPVN

• Full Name: kinesin family member 2C
• Calculated Molecular Weight: 725 aa, 81 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC014924
• Gene Symbol: KIF2C
• Gene ID (NCBI): 11004
• RRID: AB_2881127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99661
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Anti-KIF2C (Cat# 28372-1-AP) has 16 total citations spanning journals including Nature, Molecular Cell, Biochemical Pharmacology, Scientific Reports, RNA Biology, International Journal of Molecular Sciences, Frontiers in Genetics, American Journal of Cancer Research, Journal of Cancer Research and Clinical Oncology, Pharmaceuticals, Computational Biology and Chemistry, Discover Oncology, Journal of Molecular Histology, American Journal of Translational Research, and Human Mutation. Research fields center on cancer biology (hepatocellular carcinoma, glioma, breast cancer, osteosarcoma, prostate cancer, Wilms tumor), neurobiology, ferroptosis, glycolysis, and ubiquitination pathways.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Nature; Reprogramming neuroblastoma by diet-enhanced polyamine depletion; Authors - Sarah Cherkaoui
human	WB	Mol Cell; The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance; Authors - Tao Liu
	WB	Biochem Pharmacol; USP36 suppresses breast cancer cell ferroptosis by regulating the ubiquitination and stability of KIF2C; Authors - Yongqiang Chen
human	IHC	Pharmaceuticals (Basel); Identification of Key Immune and Cell Cycle Modules and Prognostic Genes for Glioma Patients through Transcriptome Analysis; Authors - Kaimin Guo
human	WB	Int J Mol Sci; KIF18B Modulates SKP2 Ubiquitination to Promote Aerobic Glycolysis and Osteosarcoma Progression.; Authors - Haonan Liu
human	IHC	Sci Rep; Bioinformatical analysis of the key differentially expressed genes for screening potential biomarkers in Wilms tumor; Authors - Linghao Cai