KIF2C Polyclonal antibody

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  WB analysis using 28372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28372-1-AP (KIF2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 28372-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28372-1-AP (KIF2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KIF2C Polyclonal Antibody for WB, IHC, ELISA

Cat No. 28372-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

KIF2C, kinesin family member 2C, Kinesin like protein 6, Kinesin like protein KIF2C, KNSL6, MCAK

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Tested Applications

• Positive WB detected in: DU 145 cells, PC-3 cells, mouse testis, rat testis
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 8 publications below
• IF: See 1 publications below

Product Information

28372-1-AP targets KIF2C in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29009

  Product name: Recombinant human KIF2C protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-186 aa of BC014924

  Sequence: MAMDSSLQARLFPGLAIKIQRSNGLIHSANVRTVNLEKSCVSVEWAEGGATKGKEIDFDDVAAINPELLQLLPLHPKDNLPLQENVTIQKQKRRSVNSKIPAPKESLRSRSTRMSTVSELRITAQENDMEVELPAAANSRKQFSVPPAPTRPSCPAVAEIPLRMVSEEMEEQVHSIRGSSSANPVN

• Full Name: kinesin family member 2C
• Calculated Molecular Weight: 725 aa, 81 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC014924
• Gene Symbol: KIF2C
• Gene ID (NCBI): 11004
• RRID: AB_2881127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99661
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Am J Transl Res; Identification of biomarkers for the transition from low-grade glioma to secondary glioblastoma by an integrated bioinformatic analysis.; Authors - Liang Zhao
human	IHC	Front Genet; KIF2C is a Biomarker Correlated With Prognosis and Immunosuppressive Microenvironment in Human Tumors.; Authors - Xiuyuan Zhang
human	WB,IHC	Am J Cancer Res; Multi-omics analysis of kinesin family member 2C in human tumors: novel prognostic biomarker and tumor microenvironment regulator; Authors - Bixi Zhang
human	IHC	J Cancer Res Clin Oncol; Development and validation of a vesicle-mediated transport-associated gene signature for predicting prognosis and immune therapy response in hepatocellular carcinoma; Authors - Zhi-Yang Liu
human	IHC	Sci Rep; Bioinformatical analysis of the key differentially expressed genes for screening potential biomarkers in Wilms tumor; Authors - Linghao Cai
human	IHC	Pharmaceuticals (Basel); Identification of Key Immune and Cell Cycle Modules and Prognostic Genes for Glioma Patients through Transcriptome Analysis; Authors - Kaimin Guo