KIF6 Polyclonal antibody

KIF6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17290-1-AP

Synonyms

C6orf102, KIF6, kinesin family member 6, Kinesin like protein KIF6



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17290-1-AP targets KIF6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF6 fusion protein Ag11164
Full Name kinesin family member 6
Calculated Molecular Weight265aa,30 kDa; 814aa,93 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC022074
Gene Symbol KIF6
Gene ID (NCBI) 221458
RRIDAB_2296657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KIF6 antibody 17290-1-APDownload protocol
IHC protocol for KIF6 antibody 17290-1-APDownload protocol
IP protocol for KIF6 antibody 17290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,monkeyWB,IF

bioRxiv

Kif6 regulates cilia motility and polarity in brain ependymal cells

Authors - Maki Takagishi
mouseIF,WB

Dis Model Mech

Motor protein Kif6 regulates cilia motility and polarity in brain ependymal cells

Authors - Maki Takagishi