KIF6 Polyclonal antibody

KIF6 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17290-1-AP

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

C6orf102, KIF6, kinesin family member 6, Kinesin like protein KIF6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis using 17290-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17290-1-AP (KIF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse testis using 17290-1-AP
IP result of anti-KIF6 (IP:17290-1-AP, 4ug; Detection:17290-1-AP 1:500) with mouse testis tissue lysate 4000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 17290-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17290-1-AP (KIF6 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 17290-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17290-1-AP (KIF6 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse testis tissue, rat testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17290-1-AP targets KIF6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11164 Product name: Recombinant human KIF6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-266 aa of BC022074 Sequence: GMREEMSLGCQEAFEIFKRDHADSVTIDDNKQILKQRFSEAKALGESINEARSKIGHLKEEITQRHIQQVALGISENMAVPLMPDQQEEKLRSQLEEEKRRYKTMFTRLKALKVEIEHLQLLMDKAKVKLQKEFEVWWAEEATNLQVNSPAVNSLDHTKPFLQTSDSQHEWSQLLSNKSSGGWEVQDQGTGRFDVCDVNARKILPSPCPSPHSQKQSSTSTPLEDSIPKRPVSSIPLTGDSQTDSDIIAFIKARQSILQKQCLGSN Predict reactive species
Full Name	kinesin family member 6
Calculated Molecular Weight	265aa,30 kDa; 814aa,93 kDa
Observed Molecular Weight	93 kDa
GenBank Accession Number	BC022074
Gene Symbol	KIF6
Gene ID (NCBI)	221458
RRID	AB_2296657
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6ZMV9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for KIF6 antibody 17290-1-AP	Download protocol
IP protocol for KIF6 antibody 17290-1-AP	Download protocol
WB protocol for KIF6 antibody 17290-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

KIF6 Polyclonal antibody (Cat# 17290-1-AP) has a total of 4 citations, published in Advanced Science, Journal of Cell Biology, Disease Models & Mechanisms, and bioRxiv. The associated research fields include cilia biology, motor protein function, mRNA transport for sperm flagellar assembly, ciliary trafficking and motility, and ependymal cell polarity.

Species	Application	Title
		Adv Sci (Weinh) The KIF6-RBP Complex Orchestrates mRNA Transport Required for Sperm Flagellar Assembly. Authors - Chunbo Xie View Article
	WB,IF	J Cell Biol Distinct roles of Kif6 and Kif9 in mammalian ciliary trafficking and motility Authors - Chuyu Fang View Article
mouse	IF,WB	Dis Model Mech Motor protein Kif6 regulates cilia motility and polarity in brain ependymal cells Authors - Maki Takagishi View Article
mouse,monkey	WB,IF	bioRxiv Kif6 regulates cilia motility and polarity in brain ependymal cells Authors - Maki Takagishi View Article