KIFC1 Recombinant antibody, PBS Only

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  WB analysis of HeLa using 85981-1-RR (same clone as 85981-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 85981-1-RR (KIFC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 85981-1-RR (same clone as 85981-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85981-1-RR (KIFC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 85981-1-RR (same clone as 85981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 85981-1-RR (same clone as 85981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 85981-1-RR (same clone as 85981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 85981-1-RR (same clone as 85981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85981-1-RR (same clone as 85981-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIFC1 antibody (85981-1-RR, Clone: 250588F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

KIFC1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 85981-1-PBS

Clone No.250588F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HSET, Kinesin-like protein 2, Kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2

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Product Information

85981-1-PBS targets KIFC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KIFC1 fusion protein Ag14492
• Full Name: kinesin family member C1
• Calculated Molecular Weight: 673 aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC000712
• Gene Symbol: KIFC1
• Gene ID (NCBI): 3833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BW19
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport (PMID: 12826589, PMID: 15236353, PMID: 23543461).