KIR2DS4/CD158i Polyclonal antibody

• 

  WB analysis of HL-60 using 21152-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21152-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21152-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 21152-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIR2DS4/CD158i antibody 21152-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

KIR2DS4/CD158i Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 21152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIR2DS4, CD158 antigen-like family member I, CD158I, Killer cell immunoglobulin-like receptor 2DS4, KIR1D

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Tested Applications

• Positive WB detected in: HL-60 cells, Jurkat cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21152-1-AP targets KIR2DS4/CD158i in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15711

  Product name: Recombinant human KIR2DS4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 21-304 aa of BC096694

  Sequence: PQEGVHRKPSFLALPGHLVKSEETVILQCWSDVMFEHFLLHREGKFNNTLHLIGEHHDGVSKANFSIGPMMPVLAGTYRCYGSVPHSPYQLSAPSDPLDMVIIGLYEKPSLSAQPGPTVQAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAVRSINGTFQADFPLGPATHGGTYRCFGSFRDAPYEWSNSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLHVLIGTSVVKIPFTILLFFLLHRWCSDKKNAAVMDQEPAGNRTVNSEDSDEQDHQEVSYA

• Full Name: killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4
• Calculated Molecular Weight: 304 aa, 34 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC096694
• Gene Symbol: KIR2DS4
• Gene ID (NCBI): 3809
• RRID: AB_10732956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43632
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIR2DS4 (also known as CD158i) is a member of the killer cell immunoglobulin-like receptor (KIR) family. KIRs are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. KIRs are mainly involved in inhibiting NK killing (inhibitory KIRs) via interaction with MHC class I molecules. KIR2DS4 has been reported to be an activating KIR and recognize the HLA-Cw4 protein.